Analyzer with automatically actuated movable closure of pipetting openings

• US 20080063567A1
• Filed: 08/31/2007
• Published: 03/13/2008
• Est. Priority Date: 09/11/2006
• Status: Abandoned Application

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Gut, Raphael, Schacher, Gottlieb
• Application Number:
  US11/897,849
• Publication Number:
  US 20080063567A1
• Time in Patent Office: Days
• US Class Current: 422/68.1
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/045 whereby the whole cover is slidable
• CPC Class Codes: B01L 2300/049 Valves integrated in closure
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: G01N 2035/00287 movable lid/cover for sample or reaction tubes
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 Reagent dispensers

Abstract

A clinical chemistry analytical apparatus for automatically analyzing a plurality of biological fluids samples is disclosed. The apparatus provides a movable cover having a pipetting opening which allows insertion of a pipetting needle therethrough, a closure mechanism which in a first state closes the pipetting opening and in a second state leaves it open, a conveyor automatically transporting a reagent container to a pipetting position, at which an opening of the reagent container is aligned with the pipetting opening, and an actuation mechanism which automatically brings the closure mechanism into the second state before introducing the pipetting needle through the pipetting opening and which brings the closure mechanism into the first state after withdrawal of the pipetting needle from the pipetting opening. The actuation mechanism is adapted to actuate the closure mechanism by a displacement of a transport device that moves the pipetting needle to and from the pipetting position.

Claims

1. A clinical chemistry analytical apparatus for automatically analyzing a plurality of samples of biological fluids, wherein aliquots of said samples are mixed with aliquots of selected reagent liquids in reaction cuvettes for forming sample-reagent-mixtures contained in a plurality of reaction cuvettes, said apparatus comprising:

an array of reagent containers and an array of reaction cuvettes, each of said reagent containers and reaction cuvettes having an opening at its upper end;

an automatic pipetting unit having a pipetting needle for taking an aliquot of reagent from one of said reagent containers and for delivering said aliquot into one of said reaction cuvettes, said automatic pipetting unit having a transport device for moving said pipetting needle to at least one pipetting position, said transport device being adapted for moving said pipetting needle along a first axis which extends in vertical direction and along a second axis which is perpendicular to said first axis;

a movable cover covering a chamber in which reagent containers are stored, said cover having a closed position at which it closes said chamber, said cover having at least one pipetting opening which allows insertion of said pipetting needle therethrough, the center of said at least one pipetting opening being located at said at least one pipetting position;

a closure mechanism mounted on said cover, said closure mechanism being adapted for being brought into a first state and into a second state, said at least one pipetting opening being closed when said closure mechanism is in said first state and said at least one pipetting opening being open when said closure mechanism is in said second state;

a conveyor configured to automatically transport each of said reagent containers to a pipetting position, at which the opening at the upper end of the reagent container is aligned with said at least one pipetting opening of said cover; and

an actuation mechanism configured to automatically actuate said closure mechanism to bring said closure mechanism into the second state before introducing said pipetting needle through said at least one pipetting opening and to automatically actuate said closure mechanism to bring said closure mechanism into the first state after withdrawal of said pipetting needle from said at least one pipetting opening,

wherein said actuation mechanism is adapted for being actuated by a displacement of said transport device associated with the motion thereof that moves said pipetting needle along said second axis to said at least one pipetting position, and respectively away from said at least one pipetting position.

2. An apparatus according to claim 1, wherein

said closure mechanism comprises a slide element which has a first permanent magnet attached thereto and which has at least one opening which allows insertion of the pipetting needle therethrough,

said slide element being in a first position when said closure mechanism is in said first state and in a second position when said closure mechanism is in said second state,

said at least one opening of the slide element being aligned with said at least one pipetting opening when said slide element is in said second position and said slide element closing said at least one pipetting opening when said slide element is in said first position,

a second permanent magnet is attached to said transport device of the pipetting needle, and

said at least one opening of the slide element, said first permanent magnet, said second permanent magnet, said pipetting needle and the path followed by the pipetting needle when it is moved by said transport device thereof are so arranged with respect to each other that

when the transport device moves the pipetting needle towards said at least one pipetting position said second permanent magnet passes close to said first permanent magnet and exerts on it a magnetic force which pulls the slide element towards its second position, and

when the transport device moves the pipetting needle away from said at least one pipetting position said second permanent magnet passes close to said first permanent magnet and exerts on it a magnetic force which pulls the slide element towards its first position.

3. An apparatus according to claim 1, wherein

said closure mechanism comprises at least one pair of slide elements which are held in a first position by springs which exert forces of opposite senses on the slide elements and press them against each other, said first position of the slide elements corresponding to said first state of said closure mechanism,

each of the slide elements of said at least one pair having a plane upper surface and a pin which extends upwards in a direction which is perpendicular to said upper surface of the slide element,

said movable cover having elongated openings each of which allows passage of a part of one of said pins therethrough and motion of said pin along said elongated opening, the end portion of said pin extending beyond the upper surface of said movable cover,

wherein said actuation mechanism is a cam attached to said transport device of the pipetting needle,

said pins of the slide elements, said cam, said pipetting needle and the path followed by the pipetting needle when it is moved by said transport device thereof are so arranged and configured with respect to each other that

when the transport device moves the pipetting needle towards said at least one pipetting position, said cam contacts the upper end portions of said pins and pushes them apart against the forces exerted by the springs and thereby brings the slide elements into a second position, said second position of the slide elements corresponding to said second state of said closure mechanism, and

when the transport device moves the pipetting needle away from said at least one pipetting position said cam moves away from the upper end portions of the slide pins and the springs push the slide elements (against each other and bring them back into their first position,

said at least one pipetting opening being closed when said slide elements are in their first position and said at least one pipetting opening being open when said slide elements are in their second position.

4. An apparatus according to claim 1, wherein

said actuation mechanism is at least one pair of solenoid actuators controlled by a controller which control the operation of said transport device of the pipetting needle, and

at least one pair of slide elements each of which is connected to the movable part of one of said solenoid actuators,

said slide elements being held in a first position by springs of said solenoid actuators in their non-operated state, said springs exerting forces of opposite senses on the slide elements and pressing them against each other, said first position of the slide elements corresponding to said first state of said closure mechanism,

said controller which control the operation of said at least two solenoid actuators and the operation of said transport device of the pipetting needle being so configured that

when the transport device moves the pipetting needle towards said at least one pipetting position and the latter reaches said position the controller operate the solenoid actuators and these pull two of the at least one pair of slide elements apart against the forces exerted by the springs of the solenoid actuators and bring the slide elements into a second position, said second position of the slide elements corresponding to said second state of said closure mechanism, and

when the transport device moves the pipetting needle away from said at least one pipetting position said controller leave said solenoid actuators non-operated and the springs of the solenoid actuators push two of the at least one pair of slide elements against each other and bring them back into their first position,

said at least one pipetting opening being closed when said slide elements are in their first position and said at least one pipetting opening being open when said slide elements are in their second position.

5. An apparatus according to claim 1, wherein said cover of said chamber is an air-tight closure thereof when it is in its closed position.

6. An apparatus according to claim 1, wherein said at least one pipetting opening is just large enough to allow insertion of said pipetting needle therethrough.

7. An apparatus according to claim 1, wherein said actuation mechanism is further configured to automatically bring said closure mechanism into the second state immediately before introducing said pipetting needle through said at least one pipetting opening and bring said closure mechanism into the first state immediately after withdrawal of said pipetting needle from said at least one pipetting opening.

8. An apparatus according to claim 1, wherein said actuation mechanism is configured to actuate said closure mechanism only by magnetic forces.