System and method for self-referencing a sensor in a micron-sized deep flow chamber
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Abstract
A system and method are described herein for self-referencing a sensor that is used to detect a biomolecular binding event and/or kinetics which occur in a sample solution flowing along side a reference solution in a micron-sized deep flow channel.
37 Citations
37 Claims
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1-12. -12. (canceled)
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13. A system comprising:
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a sensor;
a micron-sized deep flow channel in which a sample solution and a reference solution flow side-by-side to one another in a sensing region of said sensor;
an interrogation system for directing an input optical beam at said sensor and receiving an output optical beam from said sensor;
said interrogation system including a camera for converting the optical beam to an electrical signal representative of an output image;
a computer for analyzing the output image to determine a detection signal associated with the sample solution flowing in a detection region of the sensing region of said sensor and to determine a reference signal associated with the reference solution flowing in a reference region of the sensing region of said sensor; and
said computer for subtracting the reference signal from the detection signal so as to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions are reduced in the corrected detection signal. - View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A system comprising:
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a sensor;
a micron-sized deep flow channel in which a sample solution and a reference solution flow side-by-side to one another in a sensing region of said sensor;
an interrogation system for directing an input optical beam at said sensor and receiving an output optical beam from said sensor;
said interrogation system including an optical detector for converting the optical beam to separate electrical signals, one each representing a detection signal associated with the sample solution flowing in a detection region of the sensing region of said sensor and another representing a reference signal associated with the reference solution flowing in a reference region of the sensing region of said sensor; and
a computer/electrical circuit for subtracting the reference signal from the detection signal so as to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions are reduced in the corrected detection signal. - View Dependent Claims (21, 22, 23, 24, 25, 26)
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27-31. -31. (canceled)
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32. A microfluidic device comprising:
a micron-sized deep flow channel in which a sample solution and a reference solution flow side-by-side to one another in a sensing region of a sensor such that an interrogation system can direct an input optical beam at the sensor and receive an output optical beam from the sensor, wherein the output optical beam is analyzed by a computer or detector system to determine a detection signal associated with the sample solution flowing in a detection region of the sensing region of the sensor and a reference signal associated with the reference solution flowing in a reference region of the sensing region of the sensor. - View Dependent Claims (33, 34, 35, 36)
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37-42. -42. (canceled)
Specification