SURFACE CROSS-LINKED LIPIDIC PARTICLES, METHODS OF PRODUCTION AND USES THEREFOR

• US 20080063621A1
• Filed: 09/07/2007
• Published: 03/13/2008
• Est. Priority Date: 09/07/2006
• Status: Abandoned Application

• Current Assignee: Maria I.C. Gyongyossy-Issa
• Original Assignee: Canadian Blood Services
• Inventors: Kizhakkedathu, Jayachandran, Constantinescu, Iren, Gyongyossy-Issa, Maria
• Application Number:
  US11/851,671
• Publication Number:
  US 20080063621A1
• Time in Patent Office: Days
• US Class Current: 424/78.31
• CPC Class Codes: A61K 35/19 Platelets; Megacaryocytes
• CPC Class Codes: A61K 47/6911 the form being a liposome
• CPC Class Codes: A61K 9/1271 Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers liposome as conjugate A61K47/6911
• CPC Class Codes: A61K 9/1277 Processes for preparing; Proliposomes
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/02 Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
• CPC Class Codes: A61P 7/04 Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system

Abstract

A method for producing a composition of lipidic particles coated with a cross-linked surface mesh, the method comprising the steps of: (i) preparing lipidic particles comprising pharmaceutically acceptable lipids, (ii) binding hydrophilic polymer chains to the surface of the lipidic particles, and (iii) cross-linking the hydrophilic polymer chains to form the cross-linked surface mesh. Pharmaceutical compositions comprising surface modified lipidic particles prepared according to this method are also described. The lipidic particles resist fusion with red blood cells and platelets in vitro, and are amenable to further derivatization by targeting molecules for controlled release of component and contents, thus providing a new generation of drug carrier systems.

Claims

1. A method for producing a composition of lipidic particles with a cross-linked surface mesh, the method comprising the steps of:

(a) preparing lipidic particles comprising pharmaceutically acceptable lipids,

(b) binding hydrophilic polymer chains to the surface of the lipidic particles, and

(c) cross-linking the hydrophilic polymer chains to form the cross-linked surface mesh.

2. The method according to claim 1, wherein the lipidic particles in step (a) comprise liposomes, vesicles, micelles, or combinations thereof.

3. The method according to claim 1, wherein the lipidic particles in step (a) comprise liposomes.

4. The method according to claim 3, wherein the liposomes are prepared using 1,2 dipalmitoyl-sn-gycero-3-phosphoethanolamine (DPPE), 1,2 dipalmitoyl-sn-gycero-3-phosphocholine (DPPC) and cholesterol (CHOL).

5. The method according to claim 4, wherein the liposomes are prepared in a formulation having a molar ratio of about 40:

30;

30, respectively, of 1,2 dipalmitoyl-sn-gycero-3-phosphoethanolamine, 1,2 dipalmitoyl-sn-gycero-3-phosphocholine, and cholesterol.

6. The method according to claim 1, wherein the hydrophilic polymer chains in step (b) are straight-chain non-toxic polymers comprising a crosslinkable end group.

7. The method according to claim 1, wherein the hydrophilic polymer chains in step (b) comprise polyethylene glycol with an acrylate end group.

8. The method according to claim 7, wherein the molecular weight of the polyethylene glycol is about 3400 mw.

9. The method according to claim 1, wherein the cross-linking in step (c) comprises cross-linking free ends of the hydrophilic polymer chains with a cross-linker.

10. The method according to claim 9, wherein the cross-linker comprises polyethylene glycol diacrylate.

11. The method according to claim 10, wherein the polyethylene glycol diacrylate comprises polyethylene glycol with a molecular weight ranging from about 700 to about 20,000.

12. The method according to claim 10, wherein the polyethylene glycol diacrylate comprises polyethylene glycol with a molecular weight of about 6000.

13. The method according to claim 10, wherein the cross-linking is conducted in the presence of ammonium persulfate under ultraviolet light.

14. The method according to claim 13, wherein the ultraviolet light wavelength is approximately 254 nm.

15. The method according to claim 10, wherein the polyethylene glycol diacrylate is diacryl-PEG₇₀₀ at a concentration between about 15 mM and 25 mM.

16. The method according to claim 10, wherein the polyethylene glycol diacrylate is diacryl-PEG₆₀₀₀ at a concentration between about 0.5 mM and 5 mM.

17. The method according to claim 16, wherein the diacryl-PEG₆₀₀₀ is at a concentration of about 0.5 mM.

18. The method according to claim 1, wherein step (a) comprises encapsulating a drug, dye, recombinant DNA or biological molecule of interest into a liposome or vesicle.

19. The method according to claim 1, further comprising binding antigens or their representative fragments, antibodies, peptides, drugs that have cell surface receptors, hormones, biological activity modifiers, enzymes, substrates, vaccines, potential vaccines, inhibitors, antithrombotic agents, or combinations thereof;

to the surface of the lipidic particles.

20. A pharmaceutical composition comprising lipidic particles with a cross-linked surface mesh, the lipidic particles comprising:

an inner lipidic particle of pharmaceutically acceptable particle-forming lipids;

hydrophilic polymer chains linked to the surface of the lipidic particle, the hydrophilic polymer chains comprising a crosslinkable end group at free ends thereof; and

cross-linker groups linking the end groups of the hydrophilic polymer chains to form the cross-linked surface mesh.

21. A lipidic particle surface modified with a cross-linked surface mesh, the lipidic particle comprising:

an inner lipidic particle of pharmaceutically acceptable particle-forming lipids;

hydrophilic polymer chains linked to the surface of the lipidic particle, the hydrophilic polymer chains comprising a crosslinkable end group at free ends thereof; and

cross-linker groups linking the end groups of the hydrophilic polymer chains to form the cross-linked surface mesh.