Isolation of Inner Cell Mass for the Establishment of Human Embryonic Stem Cell (hESC) Lines
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Abstract
A method of establishing a cell line from the inner cell mass of a blastocyst, comprising: isolating a blastocyst having a zona pellucida, a trophectoderm, and an inner cell mass; creating an aperture in the blastocyst by laser ablation; isolating cells from the inner cell mass from the blastocyst through the aperture; and culturing the cells to establish a cell line, wherein the cells are cultured under feeder free conditions.
9 Citations
42 Claims
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1-20. -20. (canceled)
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21. A method of establishing a cell line from the inner cell mass of a human blastocyst, comprising:
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(a) isolating a blastocyst having a zona pellucida, a trophectoderm, and an inner cell mass;
(b) creating an aperture in the blastocyst by laser ablation;
(c) isolating cells from the inner cell mass from the blastocyst through the aperture; and
(d) culturing the cells to establish a cell line, wherein the cells are cultured under feeder free conditions. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A method of establishing a human embryonic stem cell line, comprising:
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(a) isolating a human blastocyst comprising an inner cell mass;
(b) creating an aperture in the blastocyst by laser ablation;
(c) isolating cells from the inner cell mass of the blastocyst through the aperture; and
(d) culturing the cells under feeder free conditions to obtain an isolated human embryonic stem cell line. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39)
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40. A method of establishing a human embryonic stem cell line, comprising:
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(a) isolating cells of an inner cell mass from a blastocyst by creating an aperture in the blastocyst by laser ablation and removing cells of the inner cell mass from the blastocyst through the aperture;
(b) culturing the cells of the inner cell mass to produce inner cell mass derived masses; and
(c) culturing the inner cell mass derived masses under feeder free conditions to produce an isolated human embryonic stem cell line. - View Dependent Claims (41, 42)
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Specification