Raman spectroscopic lateral flow test strip assays
First Claim
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1. A method for measuring at least one selected analyte in a liquid sample, comprising the steps of:
- providing a lateral flow test strip unit that includes;
a sample zone for depositing a liquid biological sample, such as a body fluid, wherein the sample zone includes, migratable SERS-active particles, coated with a first binding element, that is specific for an analyte to be measured, a capture zone having a second binding element that specifically binds to the analyte and/or epitopes presented by the analyte and bound first binding element, but not by the first-binding element alone; and
depositing a sample on the sample zone; and
after allowing sufficient time for migration of the particles through lateral flow strip, determining the presence, absence or concentration of the analyte in the sample by;
irradiating at least part of the capture zone with monochromatic light to generate a Raman spectra from particles that may be captured in the capture zone, measuring the intensity of at least part of the Raman spectra that is specific for the first binding element on particles that may have been captured in the capture zone, calculating the presence/absence or concentration of the analyte in the sample based on the measured intensity.
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Abstract
The invention provides improved Raman spectroscopy-based methods and systems for the quantitative analysis of selected analytes using lateral flow binding assay test strips.
66 Citations
14 Claims
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1. A method for measuring at least one selected analyte in a liquid sample, comprising the steps of:
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providing a lateral flow test strip unit that includes;
a sample zone for depositing a liquid biological sample, such as a body fluid, wherein the sample zone includes, migratable SERS-active particles, coated with a first binding element, that is specific for an analyte to be measured, a capture zone having a second binding element that specifically binds to the analyte and/or epitopes presented by the analyte and bound first binding element, but not by the first-binding element alone; and
depositing a sample on the sample zone; and
after allowing sufficient time for migration of the particles through lateral flow strip, determining the presence, absence or concentration of the analyte in the sample by;
irradiating at least part of the capture zone with monochromatic light to generate a Raman spectra from particles that may be captured in the capture zone, measuring the intensity of at least part of the Raman spectra that is specific for the first binding element on particles that may have been captured in the capture zone, calculating the presence/absence or concentration of the analyte in the sample based on the measured intensity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for measuring at least one selected analyte in a liquid sample, that includes the steps of:
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providing a lateral flow test strip unit that includes, a sample zone for depositing a liquid biological sample, such as a body fluid, wherein the sample zone includes migratable SERS-active particles coated with a first binding element, that is specific for an analyte to be measured a capture zone having a second binding element that specifically binds to the analyte and/or epitopes presented by the analyte and bound first binding element, but not by the first binding element alone, and a control zone having a third binding element such as an antibody that binds to the first binding element, the control zone, wherein the capture zone is located between the sample zone and the control zone;
depositing a sample on the sample zone, after allowing sufficient time for migration of the particles through lateral flow strip, determining the presence, absence or concentration of the analyte in the sample by;
irradiating at least part of the capture zone with monochromatic light to generate a Raman spectra from particles that may be captured in the capture zone, measuring the intensity of at least part of the Raman spectra that is specific for the first binding element on particles that may have been captured in the capture zone, irradiating at least part of the control zone with monochromatic light to generate a Raman spectra from particles that may be captured in the control zone, measuring the intensity of at least part of the Raman spectra that is specific for the first binding element on particles that may have been captured in the control zone. - View Dependent Claims (11, 12)
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13. A system for performing lateral flow test strip assays using Raman spectroscopy, comprising:
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a portable Raman reader unit; and
a lateral flow assay test strip unit comprising migratable SERS-active particles to which at least one analyte binding element is bound, wherein the Raman reader unit and test strip unit are mutually adapted to allignedly position the test strip unit with respect to the Raman reader unit for the reading of at least one test or control stripe of the strip by the reader unit. - View Dependent Claims (14)
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Specification