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OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS

  • US 20080068588A1
  • Filed: 11/21/2007
  • Published: 03/20/2008
  • Est. Priority Date: 05/23/2005
  • Status: Active Grant
First Claim
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1. A method of imaging with an optical system characterized by a diffraction-limited resolution volume, the method comprising:

  • in a sample comprising a plurality of phototransformable optical labels (“

    PTOLs”

    ) distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system, activating a first subset of the PTOLs in the sample, wherein the density of the activated PTOLs in the first subset is less than the inverse of the diffraction-limited resolution volume;

    exciting a first portion of the PTOLs in the first subset of PTOLs;

    detecting radiation emitted from the activated and excited PTOLs in the first portion of PTOLs with the imaging optics; and

    determining locations of activated and excited PTOLs in the first portion of PTOLs with a sub-diffraction-limited accuracy based on the detected radiation emitted from the activated and excited PTOLs.

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