Umbilical cord biomaterial for medical use

• US 20080069895A1
• Filed: 08/15/2007
• Published: 03/20/2008
• Est. Priority Date: 08/15/2006
• Status: Active Grant

• Current Assignee: Celularity, Inc.
• Original Assignee: Anthrogenesis Corporation (Bristol-Myers Squibb Company)
• Inventors: Rendon Barragan, Henry, Matcham, George, Liu, Qing
• Granted Patent: US 8,105,634 B2
• Time in Patent Office: Days
• US Class Current: 424/583
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61P 27/16 Otologicals

Abstract

The present invention provides a biomaterial comprising a mammalian umbilical cord membrane. The biomaterial can additionally comprise Wharton'"'"'s jelly and/or one or more umbilical cord vessels. The biomaterial is preferably dry, and can be flat, tubular, or shaped to fit a particular body structure. The invention further provides laminates comprising at least one layer of an umbilical cord membrane biomaterial. The invention further provides methods of making the biomaterial, and laminates comprising the biomaterial, and methods of using the biomaterial.

Claims

1. An umbilical cord-derived biomaterial comprising an isolated mammalian umbilical cord membrane and Wharton'"'"'s jelly, wherein said biomaterial comprises less than 20% water by weight.

2. The biomaterial of claim 1 that comprises at least one umbilical vessel.

3. The biomaterial of claim 1 that is decellularized.

4. The biomaterial of claim 1 comprising an exogenous bioactive molecule.

5. The biomaterial of claim 4, wherein said bioactive molecule is a cytokine or a growth factor.

6. The biomaterial of claim 4, wherein said bioactive molecule is an extracellular matrix protein.

7. The biomaterial of claim 6, wherein said extracellular matrix protein is collagen, fibronectin, elastin, vitronectin, or hyaluronic acid.

8. The biomaterial of claim 4, wherein said bioactive molecule is hyaluronic acid.

9. The biomaterial of claim 8, wherein said hyaluronic acid is crosslinked to said umbilical cord membrane.

10. The biomaterial of claim 1, comprising an exogenous polymer.

11. The biomaterial of claim 10, wherein said exogenous polymer is a synthetic biodegradable polymer or an anionic polymer.

12. The biomaterial of claim 4, wherein the bioactive molecule is an antibiotic, a hormone, a growth factor, an anti-tumor agent, an anti-fungal agent, an anti-viral agent, a pain medication, an anti-histamine, an anti-inflammatory agent, an anti-infective agent, a wound healing agent, a wound sealant, a cellular attractant, a scaffolding reagent, or a small molecule.

13. The biomaterial of claim 12, further comprising a hydrogel composition.

14. The biomaterial of claim 1, additionally comprising an exogenous stem cell.

15. The biomaterial of claim 3, additionally comprising an exogenous stem cell.

16. The biomaterial of claim 14, wherein said exogenous stem cell is a placental stem cell, a mesenchymal stem cell, an embryonic stem cell, or a somatic stem cell.

17. The biomaterial of claim 16, wherein said somatic stem cell is a neural stem cell, a hepatic stem cell, a pancreatic stem cell, an endothelial stem cell, a cardiac stem cell, or a muscle stem cell.

18. A laminate comprising a plurality of layers, wherein at least one of the layers comprises the biomaterial of claim 1.

21. A method of delivering a therapeutic agent to a subject comprising contacting the subject with the composition of claim 1, wherein said composition comprises a therapeutic agent.

19. A method of producing a biomaterial comprising isolating and decellularizing a composition comprising an umbilical cord membrane.

20. A biomaterial made by the method of claim 19.

22. A method of repairing a tympanic membrane having a deformity, comprising contacting said tympanic membrane with a biomaterial comprising an isolated mammalian umbilical cord membrane.