Compositions and Methods for Preserving Permeation Layers for Use on Active Electronic Matrix Devices

• US 20080069962A1
• Filed: 08/28/2007
• Published: 03/20/2008
• Est. Priority Date: 08/31/2006
• Status: Active Grant

• Current Assignee: Gamida for Life B.V.
• Original Assignee: Gamida for Life B.V.
• Inventors: Nguyen, Kenny, Light, James
• Granted Patent: US 7,687,103 B2
• Time in Patent Office: Days
• US Class Current: 427/384
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

An improved synthetic, polymer hydrogel permeation layer for use with an active electronic matrix device for biological assays. The permeation layer includes a dried coating of raffinose or a combination of raffinose and stachyose to protect the permeation layer from degradation during shipping and storage.

Claims

1. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of raffinose.

2. The sugar-based coating of claim 1, wherein the solution of raffinose further comprises raffinose in an amount of between about 0 to 10% by weight.

3. The sugar-based coating of claim 2, wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.

4. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of raffinose and stacyhose.

5. The sugar-based coating of claim 4, wherein the solution of raffinose and stachyose further comprises raffinose and stachyose in an amount of between about 0 to 10% by weight.

6. The sugar-based coating of claim 5, wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.

7. A method of coating a permeation layer overlying an electrode on a substrate, the method the comprising the steps of:

a. preparing a permeation layer;

b. preparing a sugar-based preservative solution;

c. covering the permeation layer with an amount of preservative solution sufficient to substantially cover an outside surface of the permeation layer; and

d. allowing preservative solution to dry on the permeation layer.

8. The method of claim 7, wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.

9. The method of claim 8, wherein the sugar-based preservative solution comprises raffinose in an amount of between about 0 to 10% by weight.

10. The method of claim 8, wherein the sugar-based preservative solution comprises raffinose and stachyose in an amount of between about 0 to 10% by weight.

11. The method of 10, wherein the sugar-based preservative solution comprises about 5% raffinose and about 5% stachyose by weight.

12. The method of 10, wherein the sugar-based preservative solution comprises about 9% raffinose and about 1% stachyose by weight.

13. The method of 10, wherein the sugar-based preservative solution comprises about 0% raffinose and about 10% stachyose by weight.