Multiple Sample Screening Using Ir Spectroscopy with Capillary Isoelectric Focusing

• US 20080074646A1
• Filed: 12/09/2005
• Published: 03/27/2008
• Est. Priority Date: 01/18/2005
• Status: Abandoned Application

• Current Assignee: Solus Biosystems, Inc.
• Original Assignee: Solus Biosystems, Inc.
• Inventors: Archibald, William
• Application Number:
  US11/795,379
• Publication Number:
  US 20080074646A1
• Time in Patent Office: Days
• US Class Current: 356/51
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/3563 for analysing solids; Preparation of samples therefor
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

A method for analyzing a fluid sample is provided which includes providing a plurality of fluid samples to be analyzed and inputting each one of the plurality of fluid samples into corresponding ones of a plurality of capillaries defined within a substrate. The method further includes applying a positive charge to each one of the plurality of fluid samples at a first end of each one of the plurality of capillaries and a negative charge to each one of the plurality of fluid samples at a second end of each one of the plurality of capillaries. The method also includes transmitting an infrared light through each one of the plurality of fluid samples at a substantially same time and detecting the infrared light transmitted through each one of the plurality of fluid samples. The method further includes generating an absorption map capable of being displayed as at least one data point based on the detection of the infrared light transmitted through each one of the plurality of fluid samples.

Claims

1-64. -64. (canceled)

65. An apparatus for analyzing aqueous fluid samples, comprising:

a sample holder having one or more infrared transparent regions, the one or more transparent regions comprising multiple etched capillary spaces with one or more sample injection ports,

a source of wide band infrared light, an infrared detector for simultaneous detection of light from multiple samples, a modulator of the wide band infrared light and a computer with a stored program for comparison of detected light signals.

66. The apparatus of claim 65, further comprising a spectral filter that limits the infrared radiation to a bandwidth of interest between 5 and 16.5 microns.

67. The apparatus of claim 65, wherein the one or more infrared transparent regions are silicon that have been microfabricated by lithography.

68. The apparatus of claim 67, wherein one infrared transparent region is held around its edge portions.

69. The apparatus of claim 67, in which deep recesses on a wafer have been prepared by deep reactive ion etch.

70. The apparatus of claim 65, comprising a Michelson interferometer for modulating the infrared light before the infrared light interacts with the sample.

71. The apparatus of claim 65, wherein the detector is a focal plane array.

72. The apparatus of claim 65, further comprising an adjustable temperature control for the sample holders.

73. The apparatus of claim 65, wherein the sample holder is a wafer with an identification marking.

74. The apparatus of claim 65, further comprising a stored program for obtaining a comparison between infrared absorbance spectra of one sample in aqueous solvent and infrared absorbance spectra of just aqueous solvent, wherein both absorbance spectra are obtained simultaneously.

75. The apparatus of claim 65, wherein the one or more infrared transparent regions comprises a semiconductor substrate made out of a material with a non-zero energy gap that separates the conduction band from the valence band.

76. A method for analyzing aqueous fluid samples, comprising:

adding a biological sample in an aqueous fluid to an infrared transparent, etched capillary space within a holder, via a sample injection port;

adding aqueous fluid to another infrared transparent, etched capillary space within the holder, via another sample injection port,

exposing both etched capillary spaces simultaneously to infrared light of a wavelength within the range 5 micron to 16.5 micron and obtaining wide range absorbance spectra from the two samples; and

obtaining a comparison signal between the samples to remove effects of water on absorbance of the biological sample

77. The method of claim 76, wherein a spectral filter is used to limit infrared radiation to a bandwidth of interest between 5 and 16.5 microns.

78. The method of claim 76, further comprising a temperature adjustment for repeatedly taking measurements at differing temperatures.

79. The method of claim 76, wherein an Michelson interferometer is used to modulate the infrared radiation before the radiation interacts with the sample.

80. The method of claim 76, wherein the etched capillary space within the holder is entirely defined within a silicon wafer.