Methods and systems for determining a baseline during image processing
First Claim
1. A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device, the method comprising:
- a) providing a baseline image;
b) providing a template image of a reaction chamber;
c) selecting a region of the baseline image;
d) performing a matching process including matching the template image to one or more portions of the region of the baseline image;
e) determining a position of a first chamber;
f) predicting a position of a second chamber; and
g) repeating steps c) through f) for subsequent chambers.
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Abstract
A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device includes a) providing a baseline image; b) providing a template image of a reaction chamber; and c) selecting a region of the baseline image. The method also includes d) performing a matching process including matching the template image to one or more portions of the region of the baseline image; e) determining a position of a first chamber; and f) predicting a position of a second chamber. The method further includes g) repeating steps c) through f) for subsequent chambers.
57 Citations
25 Claims
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1. A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device, the method comprising:
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a) providing a baseline image;
b) providing a template image of a reaction chamber;
c) selecting a region of the baseline image;
d) performing a matching process including matching the template image to one or more portions of the region of the baseline image;
e) determining a position of a first chamber;
f) predicting a position of a second chamber; and
g) repeating steps c) through f) for subsequent chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of determining positions of a plurality of chambers of a microfluidic device, the method comprising:
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providing a passive reference image of the microfluidic device;
determining a plurality of first chamber positions based on the passive reference image;
providing an additional passive reference image of the microfluidic device;
determining a plurality of second chamber positions based on the additional passive reference image;
providing a reporter dye image of the microfluidic device;
determining a plurality of third chamber positions based on the reporter dye image; and
determining one or more statistical parameters based on the reporter dye image. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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18. A method of removing a baseline from PCR ratio data, the method comprising:
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obtaining a plurality of images, each of the plurality of images associated with a cycle of a PCR process;
determining a ratio of reporter dye to passive reference dye for each of the plurality of images, thereby providing a ratio data curve;
determining a corner point of the ratio data curve;
determining a baseline value associated with the ratio data curve; and
subtracting the baseline value from each value in the ratio data curve, thereby providing a baseline removed ratio data curve. - View Dependent Claims (19, 20)
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21. A method of determining a change in threshold value for a plurality of ratio data curves, the method comprising:
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determining a first quality factor for a first ratio data curve of the plurality of ratio data curves;
determining that the first quality factor is greater than a predetermined value;
determining a second quality factor for a second ratio data curve of the plurality of ratio data curves;
determining that the second quality factor is greater than the predetermined value; and
determining the change in threshold value based on the first ratio data curve and the second ratio data curve. - View Dependent Claims (22, 23, 24, 25)
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Specification