Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
First Claim
Patent Images
1. A method for producing pluripotent (ES) cells that can be used to produce differentiated cells and tissues comprising:
- (a) obtaining a haploid cell in metaphase II that comprises DNA derived from a single individual male or female, which optionally may be genetically modified;
(b) activating said haploid cell by a method selected from the group consisting of (1) conditions that do not result in second polar body extrusion;
(2) conditions that provide for polar body extrusion but in the presence of an agent that inhibits polar body extrusion, and (3) conditions that prevent the initial cleavage, and culturing said activated cell to produce a gynogenetic or androgenetic embryo comprising a discernible trophectoderm and an inner cell mass;
(c) isolating said inner cell mass or cells therefrom and transferring said inner cell mass or cells to an in vitro media that inhibits differentiation of said inner cell mass derived therefrom; and
(d) culturing said inner cell mass cells or cells derived therefrom to maintain said cells in an undifferentiated pluripotent state.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods for obtaining pluripotent (embryonic stem) cells from parthenogenetic embryos, especially primates, are provided. These cells are useful for producing differentiated cells, tissues and organs, especially human and non-human primate cells, tissues and organs.
-
Citations
38 Claims
-
1. A method for producing pluripotent (ES) cells that can be used to produce differentiated cells and tissues comprising:
-
(a) obtaining a haploid cell in metaphase II that comprises DNA derived from a single individual male or female, which optionally may be genetically modified;
(b) activating said haploid cell by a method selected from the group consisting of (1) conditions that do not result in second polar body extrusion;
(2) conditions that provide for polar body extrusion but in the presence of an agent that inhibits polar body extrusion, and (3) conditions that prevent the initial cleavage, and culturing said activated cell to produce a gynogenetic or androgenetic embryo comprising a discernible trophectoderm and an inner cell mass;
(c) isolating said inner cell mass or cells therefrom and transferring said inner cell mass or cells to an in vitro media that inhibits differentiation of said inner cell mass derived therefrom; and
(d) culturing said inner cell mass cells or cells derived therefrom to maintain said cells in an undifferentiated pluripotent state. - View Dependent Claims (33, 34, 35)
-
-
2-16. -16. (canceled)
-
17. A method for producing pluripotent (ES) cells that can be used to desired differentiated cell types comprising:
-
(i) producing a diploid cell by implantation of two haploid nuclei derived from the same male or female individual, which optionally may be genetically modified;
(ii) gynogenetically or androgeneticaly activating said diploid metaphase II cell to produce an embryo having a discernible trophectoderm and inner cell mass; and
(iii) isolating said inner cell mass or cells therefrom and culturing said ICM or cells therefrom in an in vitro media that maintains said cells in a pluripotent, undifferentiated state. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 29, 30, 31)
-
- 26. Pluripotent cells derived from haploid cells of male or female origin.
- 36. A method of identifying growth factors that induce differentiation into specific cell types comprising exposing pluripotent cells to different combinations of growth factors, and selecting growth factor combinations that result in differentiation into specific differentiated cell types.
Specification