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Polymerase variants for DNA sequencing

  • US 20080076123A1
  • Filed: 09/27/2006
  • Published: 03/27/2008
  • Est. Priority Date: 09/27/2006
  • Status: Abandoned Application
First Claim
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1. A method for determining the sequence of a nucleic acid comprising the steps of;

  • contacting a nucleic acid duplex, comprising primer nucleic acid hybridized to a template nucleic acid with a Klenow exo

    DNA polymerase having a glycine for proline substitution at position 680 in the presence of a first labeled nucleotide under conditions that permit the polymerase to add nucleotides to said primer in a template-dependent manner, detecting a signal from the incorporated labeled nucleotide, and repeating said contacting and detecting steps at least once, wherein sequential detection of incorporated labeled nucleotide determines the sequence of the nucleic acid.

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  • 6 Assignments
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