Lipolytic Enzyme Assay
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Abstract
The present invention relates to a method for measuring the activity of a lipolytic enzyme comprising measuring the pH of the gas phase of the enzymatic reaction between the lipolytic enzyme and a substrate, wherein the substrate comprises an ester bond between a C1-C10 carboxylic acid and an alcohol. Furthermore, it relates to a method for screening a library of polypeptide for a lipolytic enzyme of interest comprising testing the library in said method.
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Citations
20 Claims
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1-10. -10. (canceled)
- 11. A method for measuring the activity of a lipolytic enzyme comprising measuring the pH of the gas phase of the enzymatic reaction between the lipolytic enzyme and a substrate, wherein the substrate comprises an ester bond between a C1-C10 carboxylic acid and an alcohol.
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12. A method for screening a library of polypeptides for a lipolytic enzyme comprising
a) measuring the pH of the gas phase of the enzymatic reaction between the polypeptides of the library and a substrate, wherein the substrate comprises an ester bond between a C1-C10 carboxylic acid and an alcohol b) selecting a lipolytic enzyme of interest.
Specification