METHOD AND APPARATUS FOR USING LIGHT TO ENHANCE CELL GROWTH AND SURVIVAL

• US 20080076836A1
• Filed: 08/22/2007
• Published: 03/27/2008
• Est. Priority Date: 09/01/2006
• Status: Abandoned Application

• Current Assignee: Cardiac Pacemakers Incorporated (Boston Scientific Corporation)
• Original Assignee: Cardiac Pacemakers Incorporated (Boston Scientific Corporation)
• Inventors: Sih, Haris, Girouard, Steven, Wagner, Darrell
• Application Number:
  US11/843,573
• Publication Number:
  US 20080076836A1
• Time in Patent Office: Days
• US Class Current: 514/789
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: G01N 2500/10 involving cells
• CPC Class Codes: G01N 33/5017 for testing neoplastic activity

Abstract

A biologic agent preparation system is provided for producing one or more biologic agents in vitro including pro-growth factors and/or pro-survival factors from cells. The one or more biologic agents are produced in response to exposure of mammalian cells to light. A biologic agent delivery system is also provided that delivers the pro-growth factors and/or pro-survival factors to an injured tissue region, or delivers cells treated with the pro-growth factors and/or pro-survival factors to the injured tissue region.

Claims

1. A method for preparing a composition comprising one or more pro-growth or pro-survival factors, comprising:

providing one or more light sources and a first population of mammalian cells in media;

exposing the first population of mammalian cells to an amount of a wavelength of light or a band of wavelengths of light for a time so as to increase the amount of one or more pro-growth factors and/or one or more pro-survival factors in the media, yielding a composition comprising the one or more pro-growth factors and/or one or more pro-survival factors; and

isolating the composition.

2. The method of claim 1, wherein isolating includes separating the media from the cells.

3. The method of claim 1, further comprising purifying the one or more pro-growth and/or pro-survival factors from the isolated composition to yield a purified composition.

4. The method of claim 3, further comprising lyophilizing the purified composition.

5. The method of claim 4, further comprising embedding the lyophilized composition in a polymeric material.

6. The method of claim 3, wherein purifying includes filtering.

7. The method of claim 1, wherein the one or more light sources emit light of 600 nm to 720 nm.

8. The method of claim 1, wherein the one or more light sources emit light of 720 nm to 1000 nm.

9. The method of claim 1, wherein one of the light sources is a light-emitting diode (LED) or an array of LEDs.

10. The method of claim 9, wherein the light sources include a plurality of LEDs and each emit light of a different wavelength or a different band of wavelengths.

11. The method of claim 1, wherein the first population comprises fibroblast cells.

12. The method of claim 1, further comprising contacting a second population of cells with the isolated composition.

13. The method of claim 12, wherein the second population of cells includes stem cells, cardiac cells or endothelial cells.

14. The method of claim 1, further comprising lyophilizing the isolated composition.

15. The method of claim 14, further comprising embedding the lyophilized composition in a polymeric material.

16. The method of claim 15, further comprising applying the polymeric material containing the lyophilized composition to at least a portion of a cardiac device or a transvascular device.

17. The method of claim 4 or 14, further comprising combining a pharmaceutically acceptable carrier with the lyophilized composition.

18. The method of claim 13, further comprising isolating the second population of cells.

19. The method of claim 18, further comprising introducing the isolated second population of cells to a device.

20. The method of claim 18, further comprising introducing the isolated second population of cells to a polymer material.

21. An isolated composition produced by the method of claim 1.

22. A purified composition produced by the method of claim 3.

23. The composition of claim 21 or 22, which is lyophilized.

24. The composition of claim 23, further comprising a pharmaceutically acceptable carrier.

34. The method of claim 1 or 25 wherein the cells are exposed to about 1000 to about 10,000 mcd or about 0.1 to about 50 mW/cm².

35. The method of claim 1 or 25 wherein the cells are exposed to about 0.1 to about 10 joules.

25. A method to identify a wavelength of light that enhances expression of pro-growth factors and/or pro-survival factors comprising:

a) contacting in vitro a first population of mammalian cells in culture media with a wavelength of light or a band of wavelengths of light;

b) collecting the media and contacting the media with a second population of mammalian cells which is different than the first population of cells; and

c) identifying whether the media enhances survival or growth of the second population of cells.

26. The method of claim 25, wherein the first population is contacted with red light.

27. The method of claim 25, wherein the first population is contacted with infrared light.

28. The method of claim 25, wherein the second population of cells comprises bone marrow cells.

29. The method of claim 25, wherein the second population of cells comprises cardiac cells.

30. The method of claim 25, wherein the second population of cells comprises endothelial cells.

31. The method of claim 25, wherein the first population of cells comprises fibroblast cells.

32. The method of claim 25, wherein the first population of cells is from a different species than the second population of cells.

33. The method of claim 32, wherein the second population of cells comprises human cells.