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Oligonucleotide probe/primer methods for polynucleotide detection

  • US 20080081335A1
  • Filed: 06/28/2007
  • Published: 04/03/2008
  • Est. Priority Date: 05/02/2005
  • Status: Abandoned Application
First Claim
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1. A method of detecting a target nucleic acid in a sample, said method comprising:

  • (a) contacting a target nucleic acid with a PCR reaction mixture comprising a labeled oligonucleotide pair having a nucleic acid primer, a nucleic acid probe and a pair of interactive labels, wherein a first member of said pair of interactive labels is coupled to said nucleic acid primer and a second member of said interactive pair of labels is coupled to said nucleic acid probe, and wherein when said primer and said probe form a hybrid said labels interact, and when said primer and probe are dissociated said labels do not interact; and

    b. permitting the cleavage of said nucleic acid probe when said nucleic acid primer is hybridized to said target nucleic acid so as to generate a detectable signal, wherein said signal is indicative of the presence of said target nucleic acid in the nucleic acid sample.

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