Oligonucleotide probe/primer methods for polynucleotide detection
First Claim
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1. A method of detecting a target nucleic acid in a sample, said method comprising:
- (a) contacting a target nucleic acid with a PCR reaction mixture comprising a labeled oligonucleotide pair having a nucleic acid primer, a nucleic acid probe and a pair of interactive labels, wherein a first member of said pair of interactive labels is coupled to said nucleic acid primer and a second member of said interactive pair of labels is coupled to said nucleic acid probe, and wherein when said primer and said probe form a hybrid said labels interact, and when said primer and probe are dissociated said labels do not interact; and
b. permitting the cleavage of said nucleic acid probe when said nucleic acid primer is hybridized to said target nucleic acid so as to generate a detectable signal, wherein said signal is indicative of the presence of said target nucleic acid in the nucleic acid sample.
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Abstract
The invention is related to a labeled oligonucleotide pair for detecting a target nucleic acid and methods, kits and compositions containing the labeled oligonucleotide pair. The labeled oligonucleotide pair forms a complex comprising a nucleic acid primer and a nucleic acid probe.
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Citations
9 Claims
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1. A method of detecting a target nucleic acid in a sample, said method comprising:
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(a) contacting a target nucleic acid with a PCR reaction mixture comprising a labeled oligonucleotide pair having a nucleic acid primer, a nucleic acid probe and a pair of interactive labels, wherein a first member of said pair of interactive labels is coupled to said nucleic acid primer and a second member of said interactive pair of labels is coupled to said nucleic acid probe, and wherein when said primer and said probe form a hybrid said labels interact, and when said primer and probe are dissociated said labels do not interact; and
b. permitting the cleavage of said nucleic acid probe when said nucleic acid primer is hybridized to said target nucleic acid so as to generate a detectable signal, wherein said signal is indicative of the presence of said target nucleic acid in the nucleic acid sample. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9)
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2. A method of detecting a target nucleic acid in a sample, said method comprising:
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a. performing a PCR amplification reaction with a PCR amplification reaction mixture comprising a target nucleic acid, and a labeled oligonucleotide pair comprising a nucleic acid primer, a nucleic acid probe which is complementary to said nucleic acid primer, and a pair of interactive labels;
b. performing a nuclease cleavage reaction; and
c. detecting a signal generated by a member of said pair of interactive labels, wherein said signal is indicative of the presence of the target nucleic acid.
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Specification