NOVEL ANTIGEN-BINDING POLYPEPTIDES AND THEIR USES
First Claim
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1. An antigen-binding polypeptide comprising one or more non-naturally encoded amino acids.
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Abstract
Novel antigen-binding polypeptides (ABP) and uses thereof are provided.
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Citations
23 Claims
- 1. An antigen-binding polypeptide comprising one or more non-naturally encoded amino acids.
- 4. An isolated nucleic acid comprising a polynucleotide that hybridizes under stringent conditions to an antigen-binding polypeptide-encoding polynucleotide, wherein the polynucleotide comprises at least one selector codon.
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9. A method of making an antigen-binding polypeptide comprising a non-naturally encoded amino acid, the method comprising, culturing cells comprising a polynucleotide or polynucleotides encoding an antigen-binding polypeptide comprising a selector codon, an orthogonal RNA synthetase and an orthogonal tRNA under conditions to permit expression of the antigen-binding polypeptide comprising a non-naturally encoded amino acid;
- and purifying the antigen-binding polypeptide.
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10. A method of modulating serum half-life or circulation time of an antigen-binding polypeptide, the method comprising substituting one or more non-naturally encoded amino acids for any one or more naturally occurring amino acids in the antigen-binding polypeptide.
- 11. An antigen-binding polypeptide encoded by a polynucleotide wherein said polynucleotide comprises a selector codon, and wherein said polypeptide comprises at least one non-naturally encoded amino acid.
- 13. A bispecific ABP comprising a first ABP and a second ABP joined to each other wherein said first ABP and said second ABP bind specifically to different epitopes wherein said first ABP has binding specificity for at least one epitope on a first antigen, and the second ABP has binding specificity for a second epitope on the first antigen or a second antigen which is different from said first epitope, and wherein said bispecific ABP comprises at least one non-naturally encoded amino acid.
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18. An antigen-binding polypeptide comprising a water soluble polymer linked by a covalent bond to the antigen-binding polypeptide at a single amino acid.
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19. An antigen-binding polypeptide comprising at least one linker, polymer, or biologically active molecule, wherein said linker, polymer, or biologically active molecule is attached to the polypeptide through a functional group of a non-naturally encoded amino acid ribosomally incorporated into the polypeptide.
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20. An antigen-binding polypeptide comprising a linker, polymer, or biologically active molecule that is attached to one or more non-naturally encoded amino acids wherein said non-naturally encoded amino acid is ribosomally incorporated into the polypeptide at pre-selected sites.
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21. A method of modulating immunogenicity of an antigen-binding polypeptide, the method comprising substituting one or more non-naturally encoded amino acids for any one or more naturally occurring amino acids in the antigen-binding polypeptide.
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22. An antigen-binding polypeptide wherein the polypeptide is selected from the group consisting of SEQ ID NO:
- 19, 21, 23, 24, 26, 28, 30, 31 or fragment thereof.
Specification