Sensor unit for assay and prism

• US 20080088847A1
• Filed: 03/19/2007
• Published: 04/17/2008
• Est. Priority Date: 03/20/2006
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Shimizu, Hitoshi, Muraishi, Katsuaki
• Granted Patent: US 7,545,501 B2
• Time in Patent Office: Days
• US Class Current: 356/446
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 2201/021 Special mounting in general

Abstract

A sensor unit is for use in a surface plasmon resonance (SPR) assay apparatus having an assay stage. A total reflection prism is supported on a stage surface of the assay stage, and has a sensing surface positioned on an upper surface thereof. The sensing surface receives illuminating light applied thereto to reflect the illuminating light. The assay apparatus receives the illuminating light reflected by the sensing surface, for measuring reaction of a sample. Two engageable ridges are disposed on first and second lateral faces of the prism which are so positioned that the sensing surface is disposed between, and keep the prism positioned on the stage surface by engagement with a retention mechanism of the assay apparatus. Furthermore, a grip portion is formed at a first end of the prism, and adapted to holding of the prism.

Claims

1. A sensor unit usable in an assay apparatus having an assay stage, comprising:

an optical block, supported on a stage surface of said assay stage, having a sensing surface positioned on an upper surface thereof, said sensing surface receiving illuminating light applied thereto to reflect said illuminating light;

said assay apparatus receiving said illuminating light reflected by said sensing surface, for measuring reaction of a sample;

at least one engageable portion of a protruding or retreating shape, formed with said optical block, for keeping said optical block positioned on said stage surface by engagement with a retention mechanism of said assay apparatus.

2. A sensor unit as defined in claim 1, wherein said at least one engageable portion is two engageable portions disposed on first and second lateral faces of said optical block which are so positioned that said sensing surface is disposed between.

3. A sensor unit as defined in claim 2, wherein said optical block is in a prismatic shape which is in an inverted trapezoidal form when viewed in a section;

said engageable portion is a ridge formed to project from a lower edge of said first and second lateral faces of said optical block extending in a block longitudinal direction.

4. A sensor unit as defined in claim 3, wherein said ridge extends consecutively in said block longitudinal direction.

5. A sensor unit as defined in claim 3, wherein said at least one engageable portion is plural engageable portions arranged in one line in said block longitudinal direction of said optical block.

6. A sensor unit as defined in claim 3, further comprising a grip portion, formed at a first end of said optical block, and adapted to holding of said optical block.

7. A sensor unit as defined in claim 1, wherein said optical block is a total reflection prism of which said upper surface is a total reflection surface.

8. A sensor unit as defined in claim 1, wherein said sensing surface is constituted by a layer of a thin film which is responsive to light applied by satisfying total reflection condition on said optical block, for attenuating intensity of reflected light thereof;

said assay apparatus is an apparatus for assay in utilizing attenuated total reflection, and includes;

a light source for applying said illuminating light to said sensing surface by satisfying total reflection condition; and

a photo detector for photoelectrically detecting said illuminating light reflected by said sensing surface.

9. A total reflection prism usable in an optical apparatus having a stage, comprising:

a prism body, shaped prismatically or semi-cylindrically, supported on a stage surface of said stage, having a total reflection surface positioned on an upper surface thereof, said total reflection surface receiving illuminating light applied thereto by a light source of said optical apparatus to reflect said illuminating light totally;

at least one engageable portion of a protruding or retreating shape, formed with said prism body, for keeping said prism body positioned on said stage surface by engagement with a retention mechanism of said optical apparatus.

10. A total reflection prism as defined in claim 9, wherein said at least one engageable portion is two engageable portions disposed on first and second lateral faces of said prism body which are so positioned that said total reflection surface is disposed between.

11. A total reflection prism as defined in claim 9, wherein said engageable portion is a ridge extending consecutively in a body longitudinal direction of said prism body.

12. A total reflection prism as defined in claim 9, wherein said at least one engageable portion is plural engageable portions arranged in one line in a body longitudinal direction of said prism body.