Retinal stem cell compositions and methods for preparing and using same
First Claim
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1. A composition comprising a population of retinal stem cells, said retinal stem cells comprising a population of reprogrammed non-retinal cells.
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Abstract
Provided are cell compositions including non-retinal cell types that have been reprogrammed to form retinal stem cells, and methods for producing and using same. Such reprogrammed cells can be used to replace one or more retinal cell types that have been lost due to damage and/or disease and are thus useful in treating or preventing visual impairment.
4 Citations
48 Claims
- 1. A composition comprising a population of retinal stem cells, said retinal stem cells comprising a population of reprogrammed non-retinal cells.
- 17. A composition comprising a population of non-retinal cell types that have been genetically altered to express or over-express a gene set comprising an eye-field transcription factor cocktail.
- 19. A composition comprising a population of non-retinal cell types that have been externally treated with one or more secreted activator or inhibitor of a signaling pathway involved in retinal stem cell formation.
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32. A method of reprogramming a population of non-retinal cells, the method comprising:
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(a) providing a cell population comprising one or more non-retinal cell types; and
(b) genetically altering the cells to express or over express a gene set comprising an eye-field transcription factor cocktail, thereby reprogramming said non-retinal cells to retinal stem cells. - View Dependent Claims (33, 34, 35)
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36. A method of reprogramming a population of non-retinal cells, the method comprising:
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a. providing a cell population comprising one or more non-retinal cell types; and
b. exposing said cells to one or more secreted activator or inhibitor of a signaling pathway involved in retinal stem cell formation. thereby reprogramming the non-retinal cells to retinal stem cells. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43)
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44. A method of reprogramming embryonic stem cells comprising:
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(a) providing a cell population of embryonic stem cells;
(b) exposing the embryonic stem cells to factors causing them to differentiate into primitive ectodermal cells; and
(c) exposing the primitive ectodermal cells to one or more secreted activator and inhibitor of a signaling pathway involved in retinal stem cell formation, thereby reprogramming said embryonic stem cells to retinal stem cells. - View Dependent Claims (45, 46, 47)
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48. A method of repopulating one or more retinal cell types, the method comprising:
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(a) providing a cell population comprising one or more non-retinal cell types;
(b) exposing the cells to one or more secreted activator or inhibitor of a signaling pathway involved in retinal stem cell formation, thereby reprogramming the non-retinal cells to retinal stem cells; and
(c) injecting the retinal stem cells of step (b) into the retina of a subject in need thereof, whereby the retinal stem cells differentiate into one or more retinal cell types thereby repopulating one or more retinal cell types that have been damaged or diseased.
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Specification