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Increased sensitivity of proximity ligation assays

  • US 20080090238A1
  • Filed: 10/12/2006
  • Published: 04/17/2008
  • Est. Priority Date: 10/12/2006
  • Status: Abandoned Application
First Claim
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1. A method for enhancing the sensitivity of a proximity ligation assay, comprising detecting and/or quantitating one or more proteins in a sample by detecting and/or quantitating one or more protein specific amplification products comprising separating at least one protein specific amplification product from a mixture of amplification products by size, wherein the mixture of amplification products is formed by amplification of a ligated probe formed when at least two different proximity probes each specifically bind the same analyte and a connector oligonucleotide binds to each of the at least two different proximity probes.

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