Increased sensitivity of proximity ligation assays

• US 20080090238A1
• Filed: 10/12/2006
• Published: 04/17/2008
• Est. Priority Date: 10/12/2006
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Peter, Brian Jon, Yang, Dan-Hui Dorothy
• Application Number:
  US11/581,191
• Publication Number:
  US 20080090238A1
• Time in Patent Office: Days
• US Class Current: 435/6.18
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2561/125 Ligase Detection Reaction [LDR]

Abstract

Methods for enhancing the sensitivity of proximity ligation assays are provided herein. The methods make use of size separation methods, control of oligonucleotide size, and control of reaction conditions, to improve the assay sensitivity. Kits for performing the assay are also described.

Claims

1. A method for enhancing the sensitivity of a proximity ligation assay, comprising detecting and/or quantitating one or more proteins in a sample by detecting and/or quantitating one or more protein specific amplification products comprising separating at least one protein specific amplification product from a mixture of amplification products by size, wherein the mixture of amplification products is formed by amplification of a ligated probe formed when at least two different proximity probes each specifically bind the same analyte and a connector oligonucleotide binds to each of the at least two different proximity probes.

2. The method of claim 1, wherein amplification of the ligated probe comprises amplifying the ligated probe with at least two primers and at least 28 amplification cycles to form the mixture of amplification products.

3. The method of claim 1, wherein each proximity probe comprises an a protein recognition moiety and an oligonucleotide probe, and wherein the at least two proximity probes specific for the same protein differ from one another by recognizing a different portion of the protein and each oligonucleotide probe of each of the at least two different proximity probes can be ligated to one another to form the ligated probe.

4. The method of claim 3, further comprising phosphorylating each oligonucleotide probe with a polynucleotide kinase before ligation of the probes.

5. The method of claim 2, wherein amplification of the ligated probe comprises PCR.

6. The method of claim 5, further comprising phosphorylating each oligonucleotide probe with a polynucleotide kinase before ligation of the probes.

7. The method of claim 1, wherein separation of the protein specific amplification product from the mixture of amplification products comprises electrophoresis.

8. The method of claim 7, wherein electrophoresis is capillary electrophoresis.

9. The method of claim 1, wherein the limit of detection for detecting and/or quantitating one or more proteins is improved by at least 2-fold as compared to a method using quantitative PCR.

10. The method of claim 1, wherein the background of detecting and/or quantitating one or more proteins is decreased by at least 2-fold as compared to a method using quantitative PCR.

11. The method of claim 2, wherein the primers provide for an amplification product of at least 100 base pairs.

12. The method of claim 11, wherein the primers provide for an amplification product of about 100 bp to about 10000 bp.

13. The method of claim 3, wherein the ligated probe is formed by hybridizing the connector oligonucleotide to each of the oligonucleotide probes of the at least two different proximity probes, wherein the connector oligonucleotide comprises a sequence complementary to each of the oligonucleotide probes and then ligating each of the oligonucleotide probes to one another using a ligase.

14. The method of claim 13, wherein the connector oligonucleotide and each oligonucleotide probe comprise chain terminating nucleotides.

15. The method of claim 14, wherein the chain-terminating nucleotides are dideoxynucleotides.

16-22. -22. (canceled)

23. A method for enhancing the sensitivity of a proximity ligation assay, comprising detecting and/or quantitating one or more analytes in a sample by detecting and/or quantitating one or more analyte specific amplification products comprising separating at least one analyte specific amplification product from a mixture of amplification products by size, wherein the mixture of amplification products is formed by amplification of a ligated probe formed when at least two different proximity probes each specifically bind the same analyte and a connector oligonucleotide binds to each of the at least two different proximity probes, wherein the one or more analytes can be detected and/or quantitated in the samples at a concentration of 0.05 pg/ml to 50 ng/ml.