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METHODS OF DETECTING LOW COPY NUCLEIC ACIDS

  • US 20080090244A1
  • Filed: 10/17/2007
  • Published: 04/17/2008
  • Est. Priority Date: 12/20/2002
  • Status: Active Grant
First Claim
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1. A method of detecting a nucleic acid of interest, the method comprising:

  • aliquotting a sample comprising the nucleic acid of interest and one or more additional nucleic acids into a plurality of reaction mixtures, wherein at least two of the reaction mixtures are single copy reaction mixtures, each comprising a single copy of the nucleic acid of interest, wherein the plurality of reaction mixtures additionally comprise at least one additional reaction mixture comprising at least one copy of the additional nucleic acid;

    subjecting the plurality of reaction mixtures to one or more amplification reactions while flowing the reaction mixtures through a channel of a microfluidic device; and

    , detecting the nucleic acid of interest in the single copy reaction mixtures.

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