Epoxide polymer surfaces

• US 20080090959A1
• Filed: 10/18/2007
• Published: 04/17/2008
• Est. Priority Date: 09/30/1997
• Status: Abandoned Application

• Current Assignee: Surmodics Incorporated
• Original Assignee: Surmodics Incorporated
• Inventors: Swan, Dale, Swanson, Melvin
• Application Number:
  US11/975,259
• Publication Number:
  US 20080090959A1
• Time in Patent Office: Days
• US Class Current: 525/54.2
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00531 essentially square
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00716 Heat activated synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07C 235/84 with the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a six-membered aromatic ring
• CPC Class Codes: C07C 323/42 Y being a carbon atom of a six-membered aromatic ring
• CPC Class Codes: C07C 45/63 : by introduction of halogen; by substitution of halogen atoms by other halogen atoms
• CPC Class Codes: C07C 49/813 : polycyclic
• CPC Class Codes: C07H 21/00 : Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C08L 63/00 : Compositions of epoxy resins; Compositions of derivatives of epoxy resins
• CPC Class Codes: C09D 4/00 : Coating compositions, e.g. paints, varnishes or lacquers, based on organic non-macromolecular compounds having at least one polymerisable carbon-to-carbon unsaturated bond ; Coating compositions, based on monomers of macromolecular compounds of groups C09D183/00 - C09D183/16
• CPC Class Codes: C12Q 1/6834 : Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: C40B 80/00 : Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers

Abstract

Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes epoxide groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.

Claims

1. A reagent composition for attaching a target molecule to the surface of a substrate, the reagent composition comprising a polymeric backbone adapted to be covalently attached to the surface and comprising one or more pendent epoxide groups adapted to form covalent bonds with corresponding functional groups on the target molecule.

2. A reagent composition according to claim 1 wherein the reagent comprises a polymer formed by the polymerization of one or more monomers selected from the group glycidyl acrylate, glycidyl methacrylate, allylglycidyl ether, and glycidyl vinyl ether.

3. A reagent composition according to claim 1 wherein the reagent comprises a polymer formed by the polymerization of one or more monomers of the formula:

4. A reagent composition according to claim 1 wherein the reagent comprises a polymer synthesized by reacting hydroxyl- or amine-containing polymers with diepoxides.

5. A reagent composition according to claim 1 wherein the target molecule comprises a nucleic acid and the surface comprises the surface of a support formed of organosilane-pretreated glass, organosilane-pretreated silicon, silicon hydride, or plastic.

6. A reagent composition according to claim 5 wherein the nucleic acid comprises an underivatized nucleic acid.

7. A reagent composition according to claim 6 wherein the underivatized nucleic acid comprises an oligonucleotide.

8. A reagent composition according to claim 1 wherein the composition further comprises one or more latent reactive groups comprising photoreactive groups for covalently attaching the reagent composition to the surface upon application of energy from a suitable source.

9. A reagent composition according to claim 8 wherein the target molecule is a nucleic acid and the photoreactive groups are selected from the group consisting of photoreactive aryl ketones.

10. A reagent composition according to claim 1 wherein the polymeric backbone is selected from the group consisting of acrylics, vinyls, nylons, polyurethanes and polyethers, and the backbone further comprises one or more pendent photoreactive groups selected from the group consisting of aryl ketones.

11-28. -28. (canceled)