Method of manufacturing hydroxyapatite and uses therefor in delivery of nucleic acids
First Claim
1. A method for making a hydroxyapatite complex, comprising reacting calcium ions with Na3PO4 in the presence of hydroxyl ions and a biomolecule, wherein the calcium ions and Na3PO4 combine to precipitate as hydroxyapatite.
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Abstract
Provided is a method for production of nanocrystalline hydroxyapatite particles, and nanocrystalline hydroxyapatite particles produced according to the method. The nanocrystalline hydroxyapatite particles exhibit substantially superior cell transformation abilities as compared to known and commercially-available calcium phosphate kits. The nanocrystalline hydroxyapatite particles also find use in tissue engineering applications, for example bone and tooth engineering and repair applications.
17 Citations
23 Claims
- 1. A method for making a hydroxyapatite complex, comprising reacting calcium ions with Na3PO4 in the presence of hydroxyl ions and a biomolecule, wherein the calcium ions and Na3PO4 combine to precipitate as hydroxyapatite.
- 16. A composition, comprising a complex of hydroxyapatite and a biomolecule wherein the hydroxyapatite has a size of 20 to 50 nanometers.
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23. A method for introducing a biomolecule into a cell, comprising contacting a cell with a composition comprising a complex of hydroxyapatite and a biomolecule wherein the complex has a size of 20 to 50 nanometers.
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