Device and Method for Detection of Analytes
First Claim
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1. A method of detecting at least one substance of interest in a liquid sample, said method comprising:
- providing a liquid sample comprising or suspected of comprising the substance(s) of interest;
applying the liquid sample to a porous material in a sufficient amount to at least partially wet the porous material;
contacting the porous material with a porous membrane comprising at least one specific binding pair member that is capable of binding, either directly or indirectly, the substance(s) of interest;
maintaining the porous material and porous membrane in contact for a sufficient amount of time for liquid present in the porous material to diffuse in, out, through, and/or about the porous membrane, wherein diffusion of the liquid in, out, through, and/or about the porous membrane results in contact of the substance(s) of interest, if present, to be bound, either directly or indirectly, to the specific binding pair member(s); and
detecting the presence or absence of a complex comprising the specific binding pair member(s) and the substance(s) of interest, wherein the presence of at least one complex indicates the presence of at least one of the substances of interest in the liquid sample.
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Abstract
The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.
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Citations
44 Claims
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1. A method of detecting at least one substance of interest in a liquid sample, said method comprising:
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providing a liquid sample comprising or suspected of comprising the substance(s) of interest;
applying the liquid sample to a porous material in a sufficient amount to at least partially wet the porous material;
contacting the porous material with a porous membrane comprising at least one specific binding pair member that is capable of binding, either directly or indirectly, the substance(s) of interest;
maintaining the porous material and porous membrane in contact for a sufficient amount of time for liquid present in the porous material to diffuse in, out, through, and/or about the porous membrane, wherein diffusion of the liquid in, out, through, and/or about the porous membrane results in contact of the substance(s) of interest, if present, to be bound, either directly or indirectly, to the specific binding pair member(s); and
detecting the presence or absence of a complex comprising the specific binding pair member(s) and the substance(s) of interest, wherein the presence of at least one complex indicates the presence of at least one of the substances of interest in the liquid sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A device for detecting at least one substance of interest in a liquid sample, the device comprising:
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(a) a receptacle comprising a porous material for receiving the liquid sample, wherein the porous material is capable of absorbing and transmitting at least a portion of the liquid sample, and (b) a porous membrane that comprises a specific binding pair member that is specific for the substance of interest or a substance bound to the substance of interest, wherein the receptacle and porous membrane are each shaped to permit the porous membrane to be in direct contact with the porous material over at least a portion of the porous membrane that comprises the specific binding pair member. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32)
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33. A device for detecting at least one substance of interest in a liquid sample, the device comprising:
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(a) a receptacle comprising a porous material for receiving the liquid sample, wherein the porous material is capable of absorbing and transmitting at least a portion of the liquid sample, and (b) a porous membrane that comprises a specific binding pair member that is specific for the substance of interest or a substance bound to the substance of interest, wherein the receptacle and porous membrane are each shaped to permit the porous membrane to be in direct contact with the porous material over at least a portion of the porous membrane that comprises the specific binding pair member, wherein the porous membrane and porous material are different elements having a different chemical constitution. - View Dependent Claims (34, 35, 36, 37)
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38. A device for detecting the presence or amount of a substance of interest in a liquid sample, the device comprising:
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a sample receiving zone for receiving the liquid sample, wherein the sample receiving zone is present on a porous material;
a sample filter zone for filtering the liquid sample received at the sample receiving zone, wherein the sample filtering zone is present on a porous material;
a porous membrane comprising a specific binding pair member at a detection zone that specifically binds to the substance of interest or a substance bound to the substance of interest, wherein the porous membrane is not the same element as any of the porous materials, and wherein the porous material and porous membrane are in physical contact over at least an area comprising a portion of the detection zone, and wherein the porous material and porous membrane are in physical contact in a configuration that permits liquid present in the liquid sample to diffuse in, out, through, and about the porous membrane in a substantially random, non-directional manner in an area comprising at least a portion of the detection zone. - View Dependent Claims (39, 40)
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41. A device comprising:
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a receptacle; and
a porous membrane comprising at least one specific binding pair member that specifically binds to at least one substance of interest or a substance bound to that substance;
wherein the receptacle comprises an application zone, a filtration zone, and a reaction zone; and
wherein the receptacle and the porous membrane are in physical contact over at least a portion of the porous membrane comprising the specific binding pair member(s) and at least a portion of the reaction zone. - View Dependent Claims (42, 43, 44)
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Specification