Method for attachment of silylated molecules to glass surfaces

• US 20080097115A1
• Filed: 01/19/2007
• Published: 04/24/2008
• Est. Priority Date: 05/28/2002
• Status: Active Grant

• Current Assignee: Nanosphere Incorporated (DiaSorin S.p.A.)
• Original Assignee: Nanosphere Incorporated (DiaSorin S.p.A.)
• Inventors: Garimella, Viswanadham, Bernal, Yasmith
• Granted Patent: US 7,482,173 B2
• Time in Patent Office: Days
• US Class Current: 556/410
• CPC Class Codes: C03C 17/30 with silicon-containing compounds
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/172307 Cyanide or isocyanide
• CPC Class Codes: Y10T 436/20 Oxygen containing

Abstract

A method for the efficient immobilization of silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.

Claims

1-79. -79. (canceled)

80. A compound having a formula vii:

[(R₁)(R₂)(R₃)Si—

X-Z-CYNH]₂Si(NHCYL-M)₂ 

 

vii

wherein R₁, R₂ and R₃ independently represents C₁-C₆ alkoxy, C₁-C₆ alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy;

L represents a linking group;

X represents linear or branched C₁-C₂₀ alkyl or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur;

Y represents oxygen or sulfur; and

Z represents oxygen or NH; and

M represents a molecule, with the proviso that at least one of R₁, R₂, or R₃ represents C₁-C₆ alkoxy.

81. The compound of claim 80 wherein L represents a nucleophilic group from the molecule.

82. The compound of claim 81 wherein the nucleophilic group comprises —

NH, —

S—

, —

O—

, or —

OOC—

.

83-87. -87. (canceled)

88. A kit comprising a compound having a formula vii:

[(R₁)(R₂)(R₃)Si—

X-Z-CYNH]₂Si(NHCYL-M)₂ 

 

vii

wherein R₁, R₂ and R₃ independently represents C₁-C₆ alkoxy, C₁-C₆ alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy;

L represents a linking group;

X represents linear or branched C₁-C₂₀ alkyl or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur;

Y represents oxygen or sulfur; and

Z represents oxygen or NH; and

M represents a molecule, with the proviso that at least one of R₁, R₂, or R₃ represents C₁-C₆ alkoxy.

89. The compound of claim 88 wherein L represents a nucleophilic group from the molecule.

90. The compound of claim 89 wherein the nucleophilic group comprises —

NH, —

S—

, —

O—

, or —

OOC—

.