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Method for measuring the concentration or change in concentration of a redox-active substance and corresponding device

  • US 20080099347A1
  • Filed: 01/26/2005
  • Published: 05/01/2008
  • Est. Priority Date: 01/29/2004
  • Status: Active Grant
First Claim
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1. Method for selecting pulse lengths for measuring the at least one of a concentration and change in concentration of a redox-active substance as a mediator in a molecular-biological detection system, in which as a result of application of suitable potentials to a working electrode, at least one of a reduction process and an oxidation process takes place as a redox reaction, the method comprising:

  • pulsing the potential of the working electrode and alternately forming measuring phases and relaxation phases;

    selecting, in this connection, the measuring-phase pulse lengths so that, towards the end of the pulse, the capacitive current is relatively small in comparison with the Faraday current; and

    selecting the relaxation-phase pulse lengths so that, towards the end of the pulse, the concentration gradient is relaxed so that at the beginning of a following measuring phase, the change in concentration of the mediator, brought about by the consumption of the mediator by the measurement itself, is reversible to the greatest possible extent.

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