Methods, Compositions and Kits Pertaining to Analyte Determination

• US 20080101989A1
• Filed: 01/07/2008
• Published: 05/01/2008
• Est. Priority Date: 01/30/2003
• Status: Active Grant

• Current Assignee: DH Technologies Pte. Ltd.
• Original Assignee: DH Technologies Pte. Ltd.
• Inventors: Bartlet-Jones, Michael, Pappin, Darryl
• Granted Patent: US 7,910,059 B2
• Time in Patent Office: Days
• US Class Current: 422/61
• CPC Class Codes: A61K 51/04 Organic compounds
• CPC Class Codes: C07D 207/46 with hetero atoms directly attached to the ring nitrogen atom
• CPC Class Codes: C07D 401/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: G01N 2458/15 Non-radioactive isotope labels, e.g. for detection by mass spectrometry
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: Y10T 436/142222 Hetero-O [e.g., ascorbic acid, etc.]
• CPC Class Codes: Y10T 436/145555 Hetero-N
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Claims

1. A kit comprising an active ester compound that is a 5, 6 or 7 membered heterocyclic ring comprising a ring nitrogen atom that is N-alkylated with a substituted or unsubstituted acetic acid moiety to which the alcohol moiety of the active ester is linked through the carbonyl carbon of the N-alkyl acetic acid moiety, wherein the compound is isotopically enriched with one or more heavy atom isotopes and further comprising one or more reagents, containers, enzymes, buffers or instructions.

2. The kit of claim 1, wherein the kit comprises a proteolytic enzyme.

3. The kit of claim 1, wherein the kit comprises two or more compounds as claimed in claim 1, wherein said two or more compounds are related as a set of isomers and/or isobaric labeling reagents.

4. The kit of claim 1, wherein each labeling reagent is independently linked to a solid support through a cleavable linker.

5. The kit of claim 1, wherein the compound has the formula: