3D MICRO-SCALE ENGINEERED TISSUE MODEL SYSTEMS
First Claim
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1. A polymeric chip, comprising, at least one porous scaffold in the chip, wherein the porous scaffold includes a first surface and a second surface, and wherein the first surface is opposite from the second surface;
- a first microfluidic inlet channel, wherein the first microfluidic inlet channel is in fluid connectivity with the first surface of the porous scaffold; and
a second microfluidic outlet channel, wherein the second microfluidic outlet channel is in fluid connectivity with the second surface of the porous scaffold.
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Abstract
A polymeric chip having at least one three-dimensional porous scaffold, a microfluidic channel inlet to the porous scaffold, and a microfluidic channel outlet from the porous scaffold. In one embodiment, the polymeric chip has two three-dimensional porous scaffolds: one scaffold comprises liver cells and the other scaffold comprises cancer cells. The chip can be used as a multi-organ tissue model system.
48 Citations
24 Claims
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1. A polymeric chip, comprising,
at least one porous scaffold in the chip, wherein the porous scaffold includes a first surface and a second surface, and wherein the first surface is opposite from the second surface; -
a first microfluidic inlet channel, wherein the first microfluidic inlet channel is in fluid connectivity with the first surface of the porous scaffold; and
a second microfluidic outlet channel, wherein the second microfluidic outlet channel is in fluid connectivity with the second surface of the porous scaffold. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A polymeric chip, comprising:
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two or more porous scaffolds in the chip, wherein each porous scaffold includes a first surface and a second surface, and wherein the first surface is opposite to the second surface;
a first microfluidic inlet channel, wherein the first microfluidic inlet channel is in fluid connectivity with the first surface of each porous scaffold; and
a second microfluidic outlet channel, wherein the second microfluidic outlet channel is in fluid connectivity with the second surface of at least one porous scaffold. - View Dependent Claims (9, 10, 11, 12)
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13. A polymeric chip, comprising,
at least one porous scaffold in the chip, wherein the porous scaffold includes a first surface and a second surface, and wherein the first surface is opposite from the second surface; -
a first microfluidic inlet channel, wherein the first microfluidic inlet channel is in fluid connectivity with the first surface of the porous scaffold; and
a second microfluidic outlet channel, wherein the second microfluidic outlet channel is in fluid connectivity with the second surface of the porous scaffold. - View Dependent Claims (14, 15)
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16. A method for assaying a compound activity, comprising,
(a) providing a polymeric chip, comprising, a first porous scaffold in the chip; -
a second porous scaffold in the chip, which is separated from the first porous scaffold;
a first microfluidic channel connecting the first porous scaffold to the second porous scaffold;
a second microfluidic channel inlet to the first porous scaffold; and
a third microfluidic channel outlet from the second porous scaffold;
(b) seeding a plurality of first living cells in the first porous scaffold to provide a first cell culture;
(c) seeding a plurality of second living cells in the second porous scaffold to provide a second cell culture;
(d) dissolving a compound in a cell culture medium to provide a liquid medium;
(e) flowing the liquid medium sequentially through the second microfluidic channel inlet, the first cell culture, the first microfluidic channel, the second cell culture, and the third microfluidic channel outlet to provide an assay solution. - View Dependent Claims (17, 18, 19)
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20. A method for assaying a compound activity, comprising,
(a) providing a polymeric chip, comprising: -
at least one porous scaffold in the chip, wherein the porous scaffold includes a first surface and a second surface, and wherein the first surface is opposite from the second surface;
a first microfluidic inlet channel, wherein the first microfluidic inlet channel is in fluid connectivity with the first surface of the porous scaffold; and
a second microfluidic outlet channel, wherein the second microfluidic outlet channel is in fluid connectivity to the second surface of the porous scaffold. (b) seeding a plurality of living cells in the porous scaffold to provide a cell culture;
(c) dissolving a compound in a cell culture medium to provide a liquid medium;
(d) flowing the liquid medium through the first microfluidic inlet channel, the cell culture, and the second microfluidic outlet channel to provide an assay solution. - View Dependent Claims (21, 22, 23, 24)
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Specification