Induction of and Maintenance of Nucleic Acid Damage in Pathogens Using Riboflavin and Light

• US 20080107636A1
• Filed: 10/31/2007
• Published: 05/08/2008
• Est. Priority Date: 06/02/2000
• Status: Active Grant

• Current Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Original Assignee: Navigant Biotechnologies, LLC
• Inventors: KEIL, Shawn, GOODRICH, Raymond
• Granted Patent: US 7,985,588 B2
• Time in Patent Office: Days
• US Class Current: 424/93.72
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 25/00 Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application , e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• CPC Class Codes: A01N 43/90 having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
• CPC Class Codes: A61K 41/17 by ultraviolet [UV] or infrared [IR] light, X-rays or gamma rays
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/0082 using chemical substances
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: A61L 2/08 Radiation
• CPC Class Codes: A61L 2/084 Visible light
• CPC Class Codes: A61L 2/10 Ultraviolet radiation
• CPC Class Codes: A61L 2202/22 Blood or products thereof
• CPC Class Codes: A61M 1/0213 with isolated sections of the tube used as additive reservoirs
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3683 using photoactive agents
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 2205/123 with incorporated reservoirs

Abstract

A process for damaging and maintaining damage to the nucleic acids of pathogens such as white blood cells, bacteria and viruses which may be contained in blood or blood components. This process comprises adding to the blood or blood component containing pathogens an effective amount of riboflavin, and exposing the fluid to light of an appropriate wavelength to damage the nucleic acid of the pathogen and to substantially maintain the damage to the pathogenic nucleic acids to allow for subsequent transfusion into a recipient.

Claims

1. A process for inactivating white blood cells which may be contained in a fluid comprising:

adding to the fluid containing white blood cells an effective amount of riboflavin;

exposing the fluid and riboflavin to light of an appropriate wavelength to activate the riboflavin and cause damage to the nucleic acid of the white blood cells; and

substantially maintaining the damage to the nucleic acids of the white blood cells to prevent re-activation of the white blood cells.

2. The process of claim 1 wherein the fluid further comprises red blood cells.

3. The process of claim 1 wherein the fluid further comprises platelets.

4. The process of claim 1 wherein the fluid further comprises plasma.

5. The process of claim 1 wherein the light to expose the fluid and riboflavin is in the UVB range.

6. The process of claim 1 wherein the riboflavin is added to the fluid at a final concentration of between about 50-500 μ

M.

7. A fluid suitable for transfusing into a patient comprising red blood cells treated by the process of claim 1.

8. A fluid suitable for transfusing into a patient comprising platelets treated by the process of claim 1.

9. A fluid suitable for transfusing into a patient comprising plasma treated by the process of claim 1.