COLLAGEN CARRIER OF THERAPEUTIC GENETIC MATERIAL AND METHOD

• US 20080107710A1
• Filed: 10/30/2007
• Published: 05/08/2008
• Est. Priority Date: 10/10/1997
• Status: Active Grant

• Current Assignee: ED. Geistlich Soehne AG Fuer Chemische Industrie
• Original Assignee: ED. Geistlich Soehne AG Fuer Chemische Industrie
• Inventors: Schloesser, Lothar, Geistlich, Peter
• Granted Patent: US 8,911,763 B2
• Time in Patent Office: Days
• US Class Current: 424/425
• CPC Class Codes: A61B 17/00234 for minimally invasive surgery A61B17/0218, A61B17/0469, A61B17/12013, A61B17/1285, A61B17/29, A61B17/320016 take precedence
• CPC Class Codes: A61B 17/00491 Surgical glue applicators surgical adhesives A61L24/00; two-component delivery syringes A61M5/19
• CPC Class Codes: A61B 17/06166 Sutures suture materials A61L17/00; manufacture of artificial threads D01D; treatment of threads D06M
• CPC Class Codes: A61F 2/0077 Special surfaces of prostheses, e.g. for improving ingrowth A61F2/30767 takes precedence
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61F 2/30767 Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
• CPC Class Codes: A61F 2002/0086 for preferentially controlling or promoting the growth of specific types of cells or tissues of bone tissue onto implantable joints A61F2002/3093
• CPC Class Codes: A61F 2002/009 for hindering or preventing attachment of biological tissue of bone tissue onto implantable joints A61F2002/30932
• CPC Class Codes: A61F 2002/2817 Bone stimulation by chemical reactions or by osteogenic or biological products for enhancing ossification, e.g. by bone morphogenetic or morphogenic proteins [BMP] or by transforming growth factors [TGF]
• CPC Class Codes: A61F 2002/30062 (bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive
• CPC Class Codes: A61F 2002/30677 Means for introducing or releasing pharmaceutical products, e.g. antibiotics, into the body
• CPC Class Codes: A61F 2002/30761 Support means for artificial cartilage, e.g. cartilage defect covering membranes
• CPC Class Codes: A61F 2002/3093 for promoting ingrowth of bone tissue
• CPC Class Codes: A61F 2002/30932 for retarding or preventing ingrowth of bone tissue
• CPC Class Codes: A61F 2002/30971 Laminates, i.e. layered products
• CPC Class Codes: A61F 2002/4635 using minimally invasive surgery
• CPC Class Codes: A61F 2210/0004 bioabsorbable
• CPC Class Codes: A61F 2240/001 Designing or manufacturing processes
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61F 2310/00982 : Coating made of collagen
• CPC Class Codes: A61K 38/1875 : Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: A61K 48/0075 : characterised by an aspect of the delivery route, e.g. oral, subcutaneous
• CPC Class Codes: A61K 9/0024 : Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61K 9/7023 : Transdermal patches and similar drug-containing composite devices, e.g. cataplasms galenical aspects of iontophoretic devices A61K9/0009; microneedle arrays A61K9/0021; buccal patches A61K9/006
• CPC Class Codes: A61L 15/325 : Collagen
• CPC Class Codes: A61L 15/40 : containing ingredients of undetermined constitution or reaction products thereof , e.g. plant or animal extracts
• CPC Class Codes: A61L 2300/258 : Genetic materials, DNA, RNA, genes, vectors, e.g. plasmids
• CPC Class Codes: A61L 2300/414 : Growth factors
• CPC Class Codes: A61L 2430/06 : for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/20 : Polysaccharides
• CPC Class Codes: A61L 27/24 : Collagen
• CPC Class Codes: A61L 27/36 : containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24
• CPC Class Codes: A61L 27/3847 : Bones
• CPC Class Codes: A61L 27/3852 : Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/54 : Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 31/005 : Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 31/044 : Collagen
• CPC Class Codes: A61L 31/14 : Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 31/16 : Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C08L 5/00 : Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
• CPC Class Codes: C08L 5/08 : Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof

Abstract

A collagen matrix material is charged with a cell growth-promoting derived nucleic acid sequence. The nucleic acid sequence-charged collagen matrix material may be utilized in a method of promoting regeneration of surface cartilage of a joint. In the method, an area of injury is covered with the nucleic acid sequence-charged collagen matrix material, the collagen matrix material is fixed over the area to be treated, and the area is allowed to heal.

Claims

1. A collagen matrix material comprised of a membrane sheet having a thickness of about 0.2-2 mm, the membrane sheet being formed from natural animal membrane comprising native insoluble collagen I, collagen III or a mixture thereof, the matrix material carrying a nucleic acid sequence encoding a protein that promotes cell growth, wherein said membrane sheet includes a barrier layer which acts as a barrier to inhibit passage of cells therethrough, wherein said barrier layer has at least one smooth face so as to inhibit cell adhesion thereon, said barrier layer further having a fibrous face opposite said smooth face.

2. The collagen matrix material of claim 1, wherein said membrane is formed from peritoneum, and has a thickness of about 0.2-0.7 mm.

3. The collagen matrix material of claim 1, comprised of a multi-layer collagen membrane, wherein said multi-layer collagen membrane comprises said membrane sheet including said barrier layer which acts as a barrier to inhibit passage of cells therethrough, wherein said multi-layer collagen membrane further comprises a matrix layer predominately of collagen II having an open sponge-like texture, wherein said matrix layer is adhered to said fibrous face.

4. The collagen matrix material of claim 3 wherein said sequence is an isolated nucleic acid sequence.

5. The collagen matrix material of claim 3 wherein said sequence is an isolated gene sequence.

6. The collagen matrix material of claim 3 wherein said sequence is an isolated DNA sequence.

7. The collagen matrix material of claim 3 wherein said sequence promotes cartilage growth.

8. The collagen matrix material of claim 3 wherein said sequence promotes bone growth.

9. A method of promoting regeneration of surface cartilage of a joint, comprising covering an area of damaged cartilage of a joint to be treated, with a collagen matrix material according to claim 1, said collagen matrix material carrying a cell growth-promoting nucleic acid sequence;

fixing said collagen matrix material over said area; and

allowing said area to regenerate cartilage.

10. The method of claim 9 wherein said collagen matrix material is comprised of a membrane comprised substantially of collagen I, collagen III or a mixture thereof.

11. The method of claim 9 wherein said collagen matrix material is a multi-layer sheet of collagen membrane material, wherein said multi-layer sheet is comprised of at least one barrier layer which acts as a barrier to inhibit passage of cells therethrough, wherein said sheet of collagen membrane material further comprises a matrix layer substantially of collagen II having an open sponge-like texture.

12. The method of claim 11 wherein said barrier layer has at least one smooth face so as to inhibit cell adhesion thereon, said barrier layer further having a fibrous face opposite said smooth face, wherein said matrix layer is adhered to said fibrous face.

13. The method of claim 11 wherein said barrier layer is comprised of collagen I, collagen III or a mixture thereof.

14. A method of repairing injury or damage to bone, cartilage or a combination thereof, comprising covering an area to be treated of injured or damaged bone, cartilage or a combination thereof, with a collagen matrix material according to claim 1, said collagen matrix material carrying a cell growth-promoting derived nucleic acid sequence;

fixing said collagen matrix material over said area; and

allowing said area to heal.

15. The method of claim 14 wherein said collagen matrix material is comprised of a membrane comprised substantially of collagen I, collagen III or a mixture thereof.

16. The method of claim 14 wherein said collagen matrix material is a multi-layer sheet of collagen membrane material, wherein said multi-layer sheet is comprised of at least one barrier layer which acts as a barrier to inhibit passage of cells therethrough, wherein said sheet of collagen membrane material further comprises a matrix layer substantially of collagen II having an open sponge-like texture.

17. The method of claim 16 wherein said barrier layer has at least one smooth face so as to inhibit cell adhesion thereon, said barrier layer further having a fibrous face opposite said smooth face, wherein said matrix layer is adhered to said fibrous face.

18. The method of claim 16 wherein said barrier layer is comprised of collagen I, collagen III or a mixture thereof.

19. The method of claim 14 wherein said area is a dental area, maxilofacial area or spinal area.

20. The method of claim 14 wherein, prior to covering said area, a cartilage defect is removed from said area.

21. The method of claim 14, wherein prior to said covering, a plurality of punctures are made to a subchondral plate of said area.