Post-synthesis processing system for supported oligonucleotides, and method

• US 20080113361A1
• Filed: 10/02/2007
• Published: 05/15/2008
• Est. Priority Date: 09/22/2004
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Vann, Charles S.
• Application Number:
  US11/906,422
• Publication Number:
  US 20080113361A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00454 by chemical cleavage from the solid support
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00542 Alphanumeric characters
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00695 Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/14 Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

A system is provided that can include: a plurality of retainment regions, where each retainment region is adapted to retain a respective type of an oligonucleotide supported on a respective support; a mixture retainment region; a handling device; a control unit adapted to control the handling device; and a separating unit adapted to simultaneously separate different supported oligonucleotides from their respective supports. A method is provided that can include: pooling together a plurality of supported oligonucleotides to form a mixture; and simultaneously separating the oligonucleotides of the supported oligonucleotides in the mixture from their supports. A method for facilitating genetic analysis is also provided and can include: receiving from a user a request for one or more genetic analysis assays; formulating each assay; and providing to the user the one or more assay; wherein the formulating can include pooling together into a mixture retainment region a plurality of different supported oligonucleotides and simultaneously separating the different oligonucleotides from their respective supports.

Claims

1. A method comprising:

pooling together a plurality of supported oligonucleotides to form a mixture, each supported oligonucleotide comprising an oligonucleotide attached to a support; and

simultaneously separating the oligonucleotides of the supported oligonucleotides in the mixture from their supports to form a pool of separated oligonucleotides.

2. The method of claim 1, further comprising packaging the pool of separated oligonucleotides in a container.

3. The method of claim 2, wherein the container is a tube or vial.

4. The method of claim 2, wherein said packaging further comprises providing information related to the pool of separated oligonucleotides.

5. The method of claim 1, wherein the plurality of supported oligonucleotides comprises a plurality of different supported oligonucleotides.

6. The method of claim 1, wherein said pooling further comprises manipulating a robot to handle respective supports for the plurality of the supported oligonucleotides while supported oligonucleotides are supported on their respective supports.

7. The method of claim 1, wherein said pooling comprises transferring the plurality of supported oligonucleotides supported on their supports to the mixture from a plurality of different respective retainment regions.

8. The method of claim 1, wherein said separating comprises chemically cleaving a plurality of different oligonucleotides from their respective support.

9. The method of claim 1, wherein the pool of separated oligonucleotides comprises a homogeneous reaction mixture.

10. The method of claim 1, wherein the homogeneous reaction mixture comprises components for conducting a fluorogenic 5′

nuclease assay.