CLIP BASED SAMPLING OF SEED FOR THE REMOVAL OF SPECIFIC SEED TISSUE OR STRUCTURES FOR SEED ANALYSIS
First Claim
1. A method of sampling seed comprising:
- a. manually positioning the seed in a pre-determined orientation;
b. removing a measurable sample from the seed by manually causing movement of one or more blades through a portion of the seed.
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Abstract
Apparatus, methods, and systems for useful sampling of seed, wherein viability is maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed using a hand-held and manually operated tool having one or more cutting edges. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is held in pre-determined orientation to facilitate efficient and accurate sampling.
107 Citations
28 Claims
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1. A method of sampling seed comprising:
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a. manually positioning the seed in a pre-determined orientation; b. removing a measurable sample from the seed by manually causing movement of one or more blades through a portion of the seed. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An apparatus for sampling an individual seed comprising:
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a. a tool comprising i. a working end including a cutting edge; ii. a handle operatively connected to the working end to cause movement of the cutting edge; b. a container for receiving a sample that is separated from the seed by the tool. - View Dependent Claims (8, 9, 10, 11)
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12. A method of sampling seed tissue from individual seed comprising:
a. separating a relatively small portion of the seed from the remainder of the seed with a hand-held and operated cutter tool wherein the separating step is adapted to provide a relatively measurable sample of the seed. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of sampling plural individual seed comprising:
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a. collecting a set of individual seed of known identification or origin; b. serially sampling individual seed from the set by; i. clipping a sample of a useful amount from a predetermined location on the seed by manually actuated cutting action; ii. without creating debris, dust, or particles; c. tracking each sample in a unique indexed location. - View Dependent Claims (23, 24, 25, 26, 27)
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28. A method of non-lethal sampling for obtaining a useful sample amount from each of a plurality of individual corn seed for physical, chemical, or genetic testing comprising:
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a. clipping from each corn seed; i. a relatively small clip; ii. from, at or near the crown and away from the embryo; iii. with a hand-held, manually-operated cutting tool; iv. without generating significant dust, debris, or contaminating material; and b. tracking each sample.
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Specification