COMPOSITIONS AND METHODS USEFUL FOR CULTURING DIFFERENTIABLE CELLS
First Claim
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1. A method of expanding differentiable cells in suspension, said method comprisinga) placing the differentiable cells in a suspension of cell medium, the medium comprising a basal salt nutrient solution and an ErbB3 ligand, where the cell medium is essentially free of serum;
- andb) maintaining the differentiable cells in the suspension of culture medium and in appropriate culture conditions for more than one day, wherein the cell medium is changed periodically,wherein the differentiable cells expand and do not differentiate.
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Abstract
The present invention relates to cell culture methods and compositions that are essentially serum-free and comprise a basal salt nutrient solution and an ErbB3 ligand.
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Citations
34 Claims
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1. A method of expanding differentiable cells in suspension, said method comprising
a) placing the differentiable cells in a suspension of cell medium, the medium comprising a basal salt nutrient solution and an ErbB3 ligand, where the cell medium is essentially free of serum; - and
b) maintaining the differentiable cells in the suspension of culture medium and in appropriate culture conditions for more than one day, wherein the cell medium is changed periodically, wherein the differentiable cells expand and do not differentiate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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19. A method of expanding differentiable cells in suspension, said method comprising
a) placing the differentiable cells in a suspension of cell medium, the medium comprising a basal salt nutrient solution and being essentially free of serum, and providing a means for stimulating ErbB2-directed tyrosine kinase activity in the differentiable cells; - and
b) maintaining the differentiable cells in the suspension of culture medium and in appropriate culture conditions for more than one day, wherein the cell medium is changed periodically, wherein the differentiable cells expand and do not differentiate. - View Dependent Claims (21)
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Specification