Simultaneous aspirator and dispenser for multiwell plates and similar devices
First Claim
1. A device for simultaneously dispensing and aspirating a sample to a testing device comprising:
- a) a body having a portion thereof adapted for engagement with the testing device;
b) dispense tubing coupled to the body for receiving a sample from a source;
c) aspirate tubing coupled to the body connected to a source of vacuum;
d) a dispense manifold connected to the dispense tubing having at least one dispensing port;
e) an aspirate manifold connected to the aspirate tubing having at least one aspirating port; and
f) a control mechanism for controlling movement of the sample from the dispense tubing to the dispense port and for simultaneously controlling application of vacuum in the aspirate tubing to the aspirating port.
3 Assignments
0 Petitions
Accused Products
Abstract
A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.
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Citations
30 Claims
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1. A device for simultaneously dispensing and aspirating a sample to a testing device comprising:
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a) a body having a portion thereof adapted for engagement with the testing device; b) dispense tubing coupled to the body for receiving a sample from a source; c) aspirate tubing coupled to the body connected to a source of vacuum; d) a dispense manifold connected to the dispense tubing having at least one dispensing port; e) an aspirate manifold connected to the aspirate tubing having at least one aspirating port; and f) a control mechanism for controlling movement of the sample from the dispense tubing to the dispense port and for simultaneously controlling application of vacuum in the aspirate tubing to the aspirating port. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of simultaneously dispensing and aspirating a sample to a testing device having at least one well, comprising the steps of:
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positioning an aspirating and dispensing device over the testing device such that an aspirating port and a dispensing port in the device are placed into the at least one well; activating a control mechanism to thereby cause a sample to enter the well via the dispensing port and simultaneously aspirating the sample from the well. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. A method of making a measurement of binding affinity of a sample, comprising the steps of:
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introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor; making a measurement of the change in the shift in peak wavelength value as a function of time (kon) from the well as the sample is introduced into the well; simultaneously aspirating and dispensing the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (koff) during the simultaneous aspirating and dispensing, calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of kon, and koff.
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Specification