Hexagonal site line scanning method and system
First Claim
1. A method for analyzing an array of discrete sites, comprising:
- simultaneously detecting a first plurality of sites in a first line of the array; and
simultaneously detecting a second plurality of sites in a second line of the array;
wherein the second line is generally parallel to the first line,wherein a distance D separates the first line and the second line when passing through nearest edges of the first and second plurality of sites, andwherein the distance between the nearest edges of adjacent sites within each of the plurality of sites is greater than D.
1 Assignment
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Accused Products
Abstract
A scanning technique for imaging sites in an array includes illuminating or irradiating sites in lines of the array, and collecting returned radiation from the sites for imaging. The sites are sequentially scanned by means of confocally directed radiation lines from source optics. The orientation of the radiation lines with respect to the lines of sites in the array is such that the distance between nearest edges of sites in adjacent lines is greater than lines through those edges in a direction parallel to the radiation lines used for scanning. The resulting system experiences less crosstalk and a greater ability to distinguish between neighboring sites in resulting images.
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Citations
35 Claims
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1. A method for analyzing an array of discrete sites, comprising:
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simultaneously detecting a first plurality of sites in a first line of the array; and simultaneously detecting a second plurality of sites in a second line of the array; wherein the second line is generally parallel to the first line, wherein a distance D separates the first line and the second line when passing through nearest edges of the first and second plurality of sites, and wherein the distance between the nearest edges of adjacent sites within each of the plurality of sites is greater than D. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for analyzing an array of discrete sites comprising:
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sequentially irradiating a series of lines of the sites, each line being irradiated with a radiation line; wherein the distance between the nearest edges of adjacent sites in each of the adjacent lines of sites is greater than the distance between parallel lines passing through the nearest edges of the sites in the adjacent lines of sites. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method for analyzing an array having discrete sites comprising:
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(a) irradiating a line of the sites with radiation; (b) repeating step (a) for a plurality of lines of sites; and (c) returning radiation from each of the lines of sites to a detector that generates signals for analysis of the sites; wherein the sites are disposed in a non-rectilinear grid on the array surface, whereby the distance between the nearest edges of adjacent sites in each line of sites is greater than the distance between parallel lines passing through the nearest edges of the sites in adjacent lines of the plurality of lines of sites, and wherein an image detected by the detector is confocal in the axis orthogonal to the axes along the parallel lines. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31)
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32. A system for analyzing an array of discrete sites, the system configured to:
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simultaneously detect a first plurality of sites in a first line of the array; and simultaneously detect a second plurality of sites in a second line of the array, wherein the second line is generally parallel to the first line, wherein a distance D separates the first line and the second line when passing through the nearest edges of the first and second plurality of sites, and wherein the distance between the nearest edges of adjacent sites within each of the plurality of sites is greater than D.
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33. A system for analyzing an array of discrete sites, the system configured to:
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sequentially irradiate a series of lines of the sites, each line being irradiated with a radiation line; wherein the distance between the nearest edges of adjacent sites in each of the adjacent lines of sites is greater than the distance between parallel lines passing through the nearest edges of the sites in the adjacent lines of sites.
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34. A system for analyzing an array of discrete sites, the system configured to:
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(a) simultaneously irradiate a plurality of sites with a line of radiation; (b) repeat step (a) for a plurality of lines of sites; and (c) return radiation from each of the pluralities of sites to a detector that generates signals for analysis of the sites; wherein the sites are disposed in a non-rectilinear grid on the array surface, wherein the distance between the nearest edges of adjacent sites in each line of sites is greater than the distance between parallel lines passing through the nearest edges of the sites in adjacent lines of the plurality of lines of sites, and wherein an image detected by the detector is confocal in the axis orthogonal to the axes along the parallel lines.
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35. A system for analyzing an array of discrete sites, the system configured to:
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(a) sequentially irradiate a plurality of sites with radiation, wherein said sites are disposed along a line, wherein the return radiation from the plurality of sites forms a line at a detector; (b) repeat step (a) for a plurality of lines of the sites; and (c) return radiation from each of the pluralities of sites to a detector that generates signals for analysis of the sites; wherein the sites are disposed in a non-rectilinear grid on the array surface, wherein the distance between the nearest edges of adjacent sites in each line of sites is greater than the distance between parallel lines passing through the nearest edges of the sites in adjacent lines of the plurality of lines of sites, and wherein an image detected by the detector is confocal in the axis orthogonal to the axes along the parallel lines.
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Specification