SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES
First Claim
1. A method of detecting multiple targets in a biological sample, comprising:
- (a) contacting the sample with a first probe;
(b) physically binding the first probe to a first target;
(c) observing a first signal from the first probe;
(d) applying a chemical agent to modify the first signal;
(e) contacting the sample with a second probe;
(f) physically binding the second probe to a second target; and
(g) observing a second signal from the second probe.
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Abstract
Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
34 Citations
32 Claims
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1. A method of detecting multiple targets in a biological sample, comprising:
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(a) contacting the sample with a first probe; (b) physically binding the first probe to a first target; (c) observing a first signal from the first probe; (d) applying a chemical agent to modify the first signal; (e) contacting the sample with a second probe; (f) physically binding the second probe to a second target; and (g) observing a second signal from the second probe. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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- 25. A kit for detection of multiple targets in a biological sample, comprising multiple probes wherein each probe capable of binding to a specific target and providing a signal and each probe is responsive to a chemical agent to result in modification of its signal.
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30. A device, comprising a sample handling system;
- a reagent dispensing system; and
a signal detection system, wherein the device is operable to detect multiple targets in a biological sample using the method comprising;(a) contacting the sample with a first probe; (b) physically binding the first probe to a first target; (c) observing a first signal from the first probe; (d) applying a chemical agent to modify the first signal; (e) contacting the sample with a second probe; (f) physically binding the second probe to a second target; and (g) observing a second signal from the second probe. - View Dependent Claims (31, 32)
- a reagent dispensing system; and
Specification