POROUS SUBSTRATE PLATES AND THE USE THEREOF
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Abstract
A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
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Citations
27 Claims
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1-14. -14. (canceled)
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15. A method for manufacturing a microplate, the method comprises:
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providing a support of glass; depositing a granular material onto said support to form a defined area of granular material; adhering individual particles of said granular material together to form a porous layer of interconnected voids attached to said support; providing a frame having a number of wells, each defined by at least a sidewall; and assembling said frame with said support to construct a microplate.
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16. A method of making a substrate used in a microplate, the method comprises the following steps:
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providing a template for forming a number of porous patches; providing a flat, rigid, non-porous understructure; and applying within said template a layer of material with granular particles to a top surface of the inorganic understructure. - View Dependent Claims (17, 18)
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19. A method for manufacturing a support plate, the method comprises:
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providing an organic or polymeric layer formed from individual granular particles that are adhered together to form a porous matrix; placing said porous layer on a understructure support plate; and attaching said porous layer to said understructure support plate by means of applying pressure and either (a) a thermal bond using a heated platen or adaptor with the configuration of a microplate, or (b) adhesive chemistry using a “
stamp”
adaptor with the same configurations of a microplate, wherein either approach (a) or (b) a section of the porous layer will be separated from other areas.
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20. A method for manufacturing a microplate, the method comprises:
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providing an understructure support of either a non-porous glass or polymer material; depositing a polymeric granular material onto a surface of said support to form a defined area of polymeric granular material; binding said polymeric granular material together to form a porous layer of interconnected voids attached to said support; providing a frame having a number of wells; and assembling said frame with said support.
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21. A method of using a microplate, the method comprises:
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providing a microplate having a number of wells, each of said wells having a three-dimensional porous-matrix located therein as a porous layer, said porous layer being either modified or unmodified with a predetermined surface chemistry for immobilizing probe species; depositing biological probes at a number of defined locations on said porous layer; and performing a bioassay with a sample. - View Dependent Claims (22, 23, 24, 25, 26, 27)
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Specification