Differentiation and identification of analogous chemical or biological substances with biosensors

• US 20080127729A1
• Filed: 10/24/2007
• Published: 06/05/2008
• Est. Priority Date: 03/25/2005
• Status: Active Grant

• Current Assignee: P.J. Edmonson Ltd.
• Original Assignee: P.J. Edmonson Ltd.
• Inventors: Edmonson, Peter J., Hunt, William D.
• Granted Patent: US 7,650,788 B2
• Time in Patent Office: Days
• US Class Current: 73/599
• CPC Class Codes: G01N 2291/0255 (Bio)chemical reactions, e.g. on biosensors
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0422 Shear waves, transverse waves, horizontally polarised waves
• CPC Class Codes: G01N 2291/0423 Surface waves, e.g. Rayleigh waves, Love waves
• CPC Class Codes: G01N 2291/0426 Bulk waves, e.g. quartz crystal microbalance, torsional waves
• CPC Class Codes: G01N 2291/0427 Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever
• CPC Class Codes: G01N 2291/045 External reflections, e.g. on reflectors
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 29/4481 Neural networks
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

An RFID biosensor detection system for detecting particular substances has an interrogator circuit to transmit a radio frequency signal, an input transducer mounted on a piezoelectric material to receive an input radio frequency signal and propagate a corresponding acoustic wave within the piezoelectric material. At least two chemically orthogonal or semi-orthogonal biolayers are mounted on the piezoelectric material to receive substances to be tested and cause corresponding changes in the acoustic wave. An output transducer is mounted on the piezoelectric material to receive the acoustic wave and transmit a corresponding output radio frequency signal. A receiver circuit receives the corresponding output radio frequency signal and provides separate channel data indicative of the substances received.

Claims

1. An RFID biosensor detection system for detecting particular substances, said system having:

an interrogator circuit to transmit a radio frequency signal;

a piezoelectric material;

an input transducer mounted on the piezoelectric material to receive an input radio frequency signal and propagate a corresponding acoustic wave within the piezoelectric material;

at least two chemically orthogonal or semi-orthogonal biolayers mounted on the piezoelectric material to receive substances to be tested and cause corresponding changes in the acoustic wave;

an output transducer mounted on the piezoelectric material to receive the acoustic wave and transmit a corresponding output radio frequency signal; and

a receiver circuit to receive the corresponding output radio frequency signal and provide separate channel data indicative of the substances received.

2. An RFID biosensor detection system according to claim 1 including a processing system to initiate an interrogation procedure.

3. An RFID biosensor detection system according to claim 1 including a processing system to effect the channel separation of data using correlation functions.

4. An RFID biosensor detection system according to claim 1 including a processing system having a state-space map formulator operable to produce a signal state-space map from the separate channel data whereby receipt of the particular substances by the biolayers of the RFID biosensor can be detected.

5. An RFID biosensor detection system according to claim 1 wherein each transducer is an interdigital transducer.

6. An RFID biosensor detection system according to claim 1 wherein each biolayer is located on an interdigital transducer.

7. An RFID biosensor detection system according to claim 1 wherein an array of reflectors causes a corresponding change in the acoustic wave.

8. An RFID biosensor detection system according to claim 7 wherein each biolayer is located on a reflector.

9. An RFID biosensor detection system according to claim 1 wherein the biolayer of each RFID biosensor device comprises an antibody, which is chemically orthogonal or semi-orthogonal to an antibody of the other biolayer.

10. A method of detecting particular substances using an RFID biosensor detection system, said method including:

providing an RFID biosensor device, such RFID biosensor device including;

an interrogator circuit to transmit a radio frequency signal;

a piezoelectric material;

an input transducer mounted on the piezoelectric material to receive an input radio frequency signal and propagate a corresponding acoustic wave within the piezoelectric material;

at least two chemically orthogonal or semi-orthogonal biolayers mounted on the piezoelectric material to receive substances to be tested and cause corresponding changes in the acoustic wave,

an output transducer mounted on the piezoelectric material to receive the acoustic wave and transmit a corresponding output radio frequency signal; and

a receiver circuit to receive the corresponding output radio frequency signal and providing separate channel data indicative of the substances received;

supplying substances to be tested to the biolayers of the RFID biosensor devices; and

utilizing the output signals from the RFID biosensor device to detect receipt of the particular substances by the biolayers of the RFID biosensor device.

11. A method according to claim 10 including producing a signal state-space map from the channel output signals to enable receipt of a particular substance by the biolayers of the RFID biosensor device to be detected.