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Detection of nucleic acids

  • US 20080131890A1
  • Filed: 06/01/2007
  • Published: 06/05/2008
  • Est. Priority Date: 12/18/2002
  • Status: Active Grant
First Claim
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1. A method for detecting an miRNA target, comprising:

  • a) providing;

    i) an miRNA target;

    ii) a first unlabled oligonucleotide that comprises a first region that is complementary to said miRNA target and a second region that is not complementary to said miRNA target;

    iii) a second unlabeled oligonucleotide that comprises a first region that is complementary to a second region of said miRNA target and a second region that is not complementary to said second region of said miRNA target;

    iv) a reverse transcriptase;

    v) a DNA polymerase; and

    vi) a probe oligonucleotide, wherein at least a portion of said probe oligonucleotide is complementary to at least a portion of said miRNA;

    b) incubating components (i) through (vi) under conditions such that a detection structure forms; and

    c) detecting said detection structure.

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