METHOD FOR MEASURING BIOLOGICAL SAMPLE AND MEASURING APPARATUS THEREFOR
First Claim
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1. A method for measuring a biological sample, comprising steps of:
- preparing a measurement sample by damaging cell membranes of erythrocytes and mature leukocytes in a biological sample, substantially not damaging cell membranes of hematopoietic progenitor cells in the biological sample, shrinking the damaged erythrocytes, and staining nucleic acid in the damaged mature leukocytes with a fluorescent dye;
irradiating cells in the measurement sample with light;
obtaining first scattered light information, second scattered light information and fluorescence information from the irradiated cells, the first scattered light information being different from the second scattered light information;
specifying hematopoietic progenitor cells based on the obtained first and second scattered light information and the obtained fluorescence information; and
counting the specified hematopoietic progenitor cells.
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Abstract
The present invention relates to a method for measuring a biological sample and a measuring apparatus therefor and in particular to a method for measuring a biological sample in which hematopoietic progenitor cells can be counted highly accurately by distinguishing them from other hemocytes and a measuring apparatus therefor.
20 Citations
17 Claims
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1. A method for measuring a biological sample, comprising steps of:
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preparing a measurement sample by damaging cell membranes of erythrocytes and mature leukocytes in a biological sample, substantially not damaging cell membranes of hematopoietic progenitor cells in the biological sample, shrinking the damaged erythrocytes, and staining nucleic acid in the damaged mature leukocytes with a fluorescent dye; irradiating cells in the measurement sample with light; obtaining first scattered light information, second scattered light information and fluorescence information from the irradiated cells, the first scattered light information being different from the second scattered light information; specifying hematopoietic progenitor cells based on the obtained first and second scattered light information and the obtained fluorescence information; and counting the specified hematopoietic progenitor cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An apparatus for measuring a biological sample, comprising:
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a means for preparing a measurement sample by damaging cell membranes of erythrocytes and mature leukocytes in a biological sample, substantially not damaging cell membranes of hematopoietic progenitor cells in the biological sample, shrinking the damaged erythrocytes, and staining nucleic acid in the damaged mature leukocytes with a fluorescent dye; a means for irradiating cells in the measurement sample with light; a means for obtaining first scattered light information, second scattered light information and fluorescence information from the irradiated cells, the first scattered light information being different from the second scattered light information; a means for specifying hematopoietic progenitor cells based on the obtained first and second scattered light information and the obtained fluorescence information; and a means for counting the specified hematopoietic progenitor cells. - View Dependent Claims (16, 17)
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Specification