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Sequencing by Incorporation

  • US 20080131904A1
  • Filed: 01/22/2008
  • Published: 06/05/2008
  • Est. Priority Date: 02/23/1999
  • Status: Active Grant
First Claim
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1. A method of sequencing a nucleic acid, comprising:

  • (i) providing a first set of particles comprising at least one set of nucleic acid templates in a first microfluidic channel;

    (ii) flowing a train of reagents comprising a plurality of sequencing reagents across the first set of particles, or flowing the first set of particles through the reagent train, thereby sequentially contacting the at least one set of nucleic acid templates with the plurality of sequencing reagents;

    (iii) detecting a signal resulting from exposure of the first set of particles to the reagent train, thereby providing a portion of sequence of the nucleic acid template; and

    ,repeating steps (ii) and (iii), thereby sequencing the nucleic acid.

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