Glucose level measuring method and glucose sensor utilizing glucose dehydrogenase
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Abstract
The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.
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Citations
32 Claims
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1-7. -7. (canceled)
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8. A glucose level measuring method using glucose dehydrogenase for measuring glucose level by utilizing a reaction system containing an enzyme and an electron carrier, the method comprising:
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using, as the enzyme, glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus; and using a Ru compound as the electron carrier. - View Dependent Claims (9, 10, 11, 12)
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13. A glucose sensor comprising:
- a first and a second electrodes; and
a reagent layer containing an enzyme and an electron carrier;the reagent layer being supplied with glucose solution to establish a reaction system, the reaction system being stimulated by using the first and the second electrodes, wherein the enzyme comprises glucose dehydrogenase to which cytochrome C is attached; and wherein the electron carrier comprises a Ru compound. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- a first and a second electrodes; and
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24. A glucose sensor comprising:
- a first and a second electrodes; and
a reagent layer containing an enzyme and an electron carrier;the reagent layer being supplied with glucose solution to establish a reaction system, the reaction system being stimulated by using the first and the second electrodes, wherein the enzyme comprises glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus; and wherein the electron carrier comprises a Ru compound. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32)
- a first and a second electrodes; and
Specification