Method For Facilitating an Automated Isolation of a Biopolymer Using Magnetic Particles
First Claim
1. A method for isolating a biopolymer from an aqueous solution utilizing magnetic particles comprising the steps of:
- a) adding magnetic particles to an aqueous solution comprising the biopolymer of interest in the presence of a salt and an additive,b) incubating the aqueous solution of step a) to allow the particles of step a) and the biopolymer of step a) to form a complex,c) applying a magnetic field to said solution to separate the magnetic particles from said solution, andd) washing the separated magnetic particles of step c) under conditions in which said biopolymer is eluted from said magnetic particles,wherein no substantial clustering of said magnetic particles occurs during the performance of the method.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method for isolating biopolymers, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from an aqueous solution utilizing magnetic particles, particularly silica magnetic particles. The method of the present invention involves forming a complex of the magnetic particles and the biopolymer in a mixture of said particles and said aqueous solution, comprising a salt and an additive; separating the complex from the mixture by a magnetic force, and eluting the biopolymer from the complex, wherein advantageously no substantial clustering of said magnetic particles occurs during the performance of the method. In a preferred embodiment the method of the invention is performed as an automated process.
-
Citations
32 Claims
-
1. A method for isolating a biopolymer from an aqueous solution utilizing magnetic particles comprising the steps of:
-
a) adding magnetic particles to an aqueous solution comprising the biopolymer of interest in the presence of a salt and an additive, b) incubating the aqueous solution of step a) to allow the particles of step a) and the biopolymer of step a) to form a complex, c) applying a magnetic field to said solution to separate the magnetic particles from said solution, and d) washing the separated magnetic particles of step c) under conditions in which said biopolymer is eluted from said magnetic particles, wherein no substantial clustering of said magnetic particles occurs during the performance of the method. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
Specification