Biomarkers and detection methods for gastric diseases

• US 20080138806A1
• Filed: 01/29/2007
• Published: 06/12/2008
• Est. Priority Date: 12/08/2006
• Status: Abandoned Application

• Current Assignee: National Taiwan University (Government of The Republic of China)
• Original Assignee: National Taiwan University (Government of The Republic of China)
• Inventors: Lin, Yu-Fen, Chow, Lu-Ping, Lin, Jaw-Town, Wu, Ming-Shiang
• Application Number:
  US11/698,820
• Publication Number:
  US 20080138806A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07K 14/205 from Campylobacter (G)
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2333/205 from Campylobacter (G)
• CPC Class Codes: G01N 33/57446 of stomach or intestine

Abstract

The present invention provides a biomarker for detecting gastric diseases, especially gastric cancer selected from: a nucleic acid sequence of GroES, complementary strand, or derivatives thereof or an amino acid sequence of GroES, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof, yet provides a kit for detecting gastric cancer by use of above-mentioned biomarkers and a detection method.

Claims

1. A biomarker for detecting gastric diseases selected from:

a nucleic acid sequence of GroES, complementary strand, or derivatives thereof or an amino acid sequence of GroES, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof.

2. The biomarker as claimed in claim 1, wherein said GroES is a specific protein of H. pylori.

3. The biomarker as claimed in claim 1, wherein said nucleic acid sequence of GroES is SEQ ID NO:

1.

4. The biomarker as claimed in claim 1, wherein said amino acid sequence of GroES is SEQ ID NO:

2.

5. The biomarker as claimed in claim 1, wherein said variants have more than 80% similarity with the amino acid sequence of SEQ ID NO:

2.

6. The biomarker as claimed in claim 1, wherein said derivatives means the nucleic acid or the complementary strand which 3′

or 5′

terminal was modified with other nucleic acid to show sequence homology with SEQ ID NO;

1 greater than 90%.

7. The biomarker as claimed in claim 1, wherein said gastric diseases is gastric cancer.

8. A biomarker for detecting gastric diseases selected from:

a nucleic acid sequence of SEQ ID NO;

1, complementary strand, derivatives thereof or an amino acid sequence of SEQ ID NO;

2, derivatives, fragments, variants thereof or antibodies against said amino acid sequences or combinations thereof.

9. The biomarker as claimed in claim 8, wherein said variants have more than 80% similarity with the amino acid sequence of SEQ ID NO:

2.

10. The biomarker as claimed in claim 8, wherein said derivatives means the nucleic acid or the complementary strand which 3′

or 5′

terminal was modified with other nucleic acid to show sequence homology greater than 90%.

11. A kit for detecting gastric disease, comprising a biomarker selected from:

a nucleic acid sequence of GroES, complementary strand or derivatives thereof or an amino acid sequence of GroES, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof.

12. The kit as claimed in claim 11, wherein said GroES is a specific protein of H. pylori.

13. The kit as claimed in claim 11, wherein sequence in said nucleic acid of GroES is SEQ ID NO:

1.

14. The kit as claimed in claim 11, wherein said amino acid sequence of GroES is SEQ ID NO:

2.

15. The kit as claimed in claim 11, further comprising a second antibody which can recognize any amino acid sequences of SEQ ID NO:

2, derivatives, fragments, variants thereof or secondary antibodies against said amino acid sequences or combinations thereof.

16. A method for detecting gastric cancer, comprising

(a) providing samples;

(b) providing biomarkers, selected from;

a nucleic acid showing SEQ ID NO;

1, complementary strand, or derivatives thereof or an amino acid sequence showing SEQ ID NO;

2, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof;

(c) contacting said biomarkers with said samples, wherein said analytes selected from;

a nucleic acid sequence of GroES, complementary strand or derivatives thereof or an amino acid sequence of GroES, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof;

(d) detecting products which result from said biomarkers contacting with said analytes in said step (c).

17. The method as claimed in claim 16, wherein said samples are serum, saliva or stomach tissue.

18. The method as claimed in claim 16, wherein said biomarkers are further immobilized on substrate.

19. The method as claimed in claim 18, wherein said substance is membrane, microplates or biochips.

20. The method as claimed in claim 16, wherein said analytes in said samples are further labelled with fluorescence markers.

21. The method as claimed in claim 16, further comprising a step for utilizing secondary antibody to recognize corresponding antibody before step (d).

22. The method as claimed in claim 16, wherein said detecting product in step (d) by means of ELISA (enzyme-linked immunosorbent assay), RIA (radioimmunoassay), western blot or immunofluorescence assay.

23. The method as claimed in claim 16, wherein said detecting product in step (d) by means of RT-PCR (reverse transcriptase-polymerase chain reaction) or in situ hybridization.

24. A biomarker for detecting gastric cancer, wherein said biomarker is selected from:

an amino acid sequence of GroES, derivatives, fragments, variants thereof or the antibodies against said amino acid sequences or combinations thereof.

25. The biomarker as claimed in claim 24, wherein said GroES is a specific protein of H. pylori.

26. The biomarker as claimed in claim 24, wherein said amino acid sequence of GroES is SEQ ID NO:

2.

27. The biomarker as claimed in claim 24, wherein said variants have more than 80% similarity with the amino acid sequence of SEQ ID NO:

2.

28. A kit for detecting gastric cancer, comprising a biomarker selected from:

an amino acid sequence of GroES, derivatives, fragments, variants thereof or the antibodies against said amino acid sequences or combinations thereof, wherein said GroES is a specific protein of H. pylori.

29. The kit as claimed in claim 28, wherein said amino acid sequence of GroES is SEQ ID NO:

2.

30. The kit as claimed in claim 28, further comprising a second antibody which can recognize any amino acid sequences of SEQ ID NO:

2, derivatives, fragments, variants thereof or antibodies against said amino acid sequences or combinations thereof.