CULTURES OF GFAP+ NESTIN+ CELLS THAT DIFFERENTIATE TO NEURONS
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Abstract
Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. The cells can be proliferated in adherent or suspension cultures. Depending on the culture conditions, the cells can be induced to differentiate to neurons or glial cells. The cultures can be expanded over a large number of passages during several months, and survive, express an astroglial phenotype and integrate well after transplantation into both neonatal and adult rat forebrain.
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Citations
85 Claims
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1-56. -56. (canceled)
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57. An in vitro cell culture of RC-2+ cells, wherein
a) one or more cells in the culture have the capacity to differentiate into neurons; -
b) the cell culture divides in a culture medium containing at least one proliferation-inducing growth factor; and c) one or more cells in the culture differentiate into neurons upon withdrawal of the proliferation-inducing growth factor. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80)
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81. The cell culture of claim 81, wherein the RC-2+ nestin+ cells show glial morphology.
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82. An in vitro adhesion cell culture of GFAP+ cells, wherein
a. one or more cells in the culture have the capacity to differentiate into neurons; -
b. the cell culture divides in a culture medium containing serum and at least one proliferation-inducing growth factor; and c. one or more cells in the culture differentiate into neurons upon withdrawal of both serum and the proliferation inducing growth factor. - View Dependent Claims (83)
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84. An in vitro cell culture essentially of:
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a. a culture medium containing serum and at least one proliferation-inducing growth factor; and b. cells derived from the central nervous system of a mammal, wherein the cells in the culture are; i. glial fibrillary acidic protein immunoreactive (GFAP+), ii. capable of proliferating in a culture medium containing serum and at least one proliferation-inducing growth factor, and iii. capable of differentiating into neurons in the absence of both the serum and the proliferation-inducing growth factor from the culture medium - View Dependent Claims (85)
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Specification