METHOD FOR ANALYZING BLOOD CONTENT OF CYTOLOGICAL SPECIMENS
First Claim
1. A method of processing a cytological specimen suspended in a liquid, comprising:
- directing light at a first wavelength through the liquid, the first wavelength being less than 450 nm;
directing light at a second wavelength longer than the first wavelength through the liquid;
detecting an intensity of light at the first wavelength transmitted through the liquid;
detecting an intensity of light at the second wavelength transmitted through the liquid;
calculating a ratio of the detected first and second wavelength light intensities;
comparing the ratio to a pre-determined threshold; and
based on the comparison, determining whether the blood content of the cytological specimen suspended in the liquid should be reduced before preparing a cell sample slide.
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Accused Products
Abstract
Methods for processing a cytological specimen suspended in a liquid. Light at a wavelength less than 450 nm is directed through the liquid. The intensity of the light transmitted through the liquid is detected and compared to a threshold to determine whether the blood content of the specimen should be reduced before a specimen slide is prepared. In one embodiment, Light at different wavelengths is directed through the liquid. One of the wavelengths is at or near a hemoglobin absorption peak that is less than 450 nm. The respective intensities of light at the different wavelengths that are transmitted through the liquid are detected, and a ratio of the detected intensities is calculated and compared to a threshold to determine whether the blood content of the specimen should be reduced before a specimen slide is prepared
92 Citations
28 Claims
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1. A method of processing a cytological specimen suspended in a liquid, comprising:
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directing light at a first wavelength through the liquid, the first wavelength being less than 450 nm; directing light at a second wavelength longer than the first wavelength through the liquid; detecting an intensity of light at the first wavelength transmitted through the liquid; detecting an intensity of light at the second wavelength transmitted through the liquid; calculating a ratio of the detected first and second wavelength light intensities; comparing the ratio to a pre-determined threshold; and based on the comparison, determining whether the blood content of the cytological specimen suspended in the liquid should be reduced before preparing a cell sample slide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of processing a cytological specimen suspended in a liquid held in a container, the method comprising:
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directing light having a wavelength less than 450 nm through the container; detecting the intensity of light transmitted through the container; comparing the intensity to a pre-determined threshold; and based on the comparison, determining whether the blood content of the cytological specimen should be reduced before preparing a slide containing cells of the cytological specimen. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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24. A method of processing a cytological specimen suspended in a liquid held in a container, comprising:
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directing light having a wavelength less than 450 nm through a cytological specimen having blood and cells; directing the light through liquid that does not include blood; detecting a first intensity of the light transmitted through the cytological specimen having blood and cells; detecting a second intensity of the light transmitted through the liquid that does not include blood; and comparing the first and second intensities to determine whether the blood content of the cytological specimen should be reduced before preparing a slide containing cells of the cytological specimen. - View Dependent Claims (25, 26, 27)
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28. A method of processing a cytological specimen suspended in a liquid held in a container, the method comprising:
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directing light at a first wavelength of about 405 nm from a first light emitting diode through the container; directing light at a second wavelength from a second light emitting diode through the container, the second wavelength being longer than the first wavelength; detecting the intensity of light at the first wavelength transmitted through the container; detecting the intensity of light at the second wavelength transmitted through the container, the first and second intensities being detected without using a spectrophotometer; calculating a ratio of the first and second intensities; comparing the ratio to a pre-determined threshold; determining, based on the comparison, whether the blood content of the cytological specimen should reduced before a slide containing cells of the cytological specimen is prepared based on the comparison; and if a determination is made that blood content should be reduced before slide preparation, treating the cytological specimen with glacial acetic acid to reduce the blood content.
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Specification