METHODS FOR IDENTIFICATION OF SEPSIS-CAUSING BACTERIA
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compositions, kits and methods for rapid identification and quantification of sepsis-causing bacteria by molecular mass and base composition analysis.
19 Citations
111 Claims
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1-55. -55. (canceled)
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56. A purified oligonucleotide primer pair comprising a forward primer and a reverse primer, each independently between 13 and 35 linked nucleotides in length, said primer pair configured to generate an amplification product between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region defined by nucleotides 649 to 783 of the reverse complement of nucleotide residues 3448565 to 3449386 of Genbank gi number:
- 49175990, and said reverse primer configured to hybridize with at least 70% complementarity to said second portion of said region.
- View Dependent Claims (57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111)
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72. A purified oligonucleotide primer pair comprising a forward primer and a reverse primer, wherein
a. said primers individually comprise between 13 and 35 linked nucleotides, b. said forward primer has at least 70% sequence identity with SEQ ID NO: - 309 and
c. said reverse primer has at least 70% sequence identity with SEQ ID NO;
1458. - View Dependent Claims (73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85)
- 309 and
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86. A kit for identifying a sepsis-causing bacterium, comprising:
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i) a first purified oligonucleotide primer pair comprising a forward primer and a reverse primer, each independently between 13 and 35 linked nucleotides in length, said primer pair configured to generate an amplification product that is between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region defined by nucleotides 649 to 783 of the reverse complement of nucleotide residues 3448565 to 3449386 of Genbank gi number;
49175990, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region; andii) at least one additional purified primer pair configured to hybridize to a bacterial gene selected from the group consisting of;
16S rRNA, 23S rRNA, tufB, rpoB, valS, rplB, and gyrB. - View Dependent Claims (87, 88, 89, 90)
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91. A method for identifying a sepsis-causing bacterium in a sample, comprising:
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a) amplifying a nucleic acid from said sample using an oligonucleotide primer pair comprising a forward primer and a reverse primer, each independently between 13 and 35 linked nucleotides in length, said primer pair configured to generate an amplification product that is between 45 and 200 linked nucleotides in length, said forward primer configured to hybridize with at least 70% complementarity to a first portion of a region defined by nucleotides 649 to 783 of the reverse complement of nucleotide residues 3448565 to 3449386 of Genbank gi number;
49175990, and said reverse primer configured to hybridize with at least 70% complementarity to a second portion of said region; andb) determining the molecular mass of said at least one amplification product by mass spectrometry. - View Dependent Claims (92, 93, 94, 95, 96, 97, 98, 99, 100)
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Specification