DEVICE, SYSTEM AND METHOD FOR IN VIVO ANALYSIS

• US 20080146896A1
• Filed: 07/31/2007
• Published: 06/19/2008
• Est. Priority Date: 01/31/2005
• Status: Active Grant

• Current Assignee: Given Imaging Limited (Medtronic Plc)
• Original Assignee: Given Imaging Limited (Medtronic Plc)
• Inventors: Rabinowitz, Elisha, Katz, Emil-Israel
• Granted Patent: US 8,738,106 B2
• Time in Patent Office: Days
• US Class Current: 600/309
• CPC Class Codes: A61B 1/041 Capsule endoscopes for imaging
• CPC Class Codes: A61B 5/073 Intestinal transmitters
• CPC Class Codes: A61K 49/0002 General or multifunctional contrast agents, e.g. chelated agents
• CPC Class Codes: A61K 49/0084 liposome, i.e. bilayered vesicular structure
• CPC Class Codes: A61K 49/0093 Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle

Abstract

A device for in vivo analysis includes: a reaction chamber to store a detecting reagent able to react with a sample collected in vivo; and optionally a labeled-substance chamber to store a labeled substance able to bind to at least a portion of a compound resulting from a reaction of the detecting reagent and the sample. The in-vivo imaging device, typically an autonomous capsule, may have a housing, the housing comprising a window; an illumination source located within the housing to illuminate a body lumen through the window; an imager to receive light reflected from the body lumen through the window; and a transmitter to transmit image data to a receiving system. The window is coated with liposomes containing a marker such that the imager may acquire images which include the marking.

Claims

1. An in vivo device to detect the presence of an analyte in a fluid sample comprising:

an inlet for obtaining the fluid sample in vivo;

a reaction chamber in which a detecting reagent reacts with the analyte in the fluid sample;

a waste chamber;

a gate to fluidically connect the reaction chamber to the waste chamber; and

an imager on board said in vivo device.

2. The in vivo device according to claim 1 wherein at least part of the reaction chamber is transparent and is in an optical path of the imager.

3. The in vivo device according to claim 1 wherein the imager is a CMOS imager, a CCD imager or a photo-detector.

4. The in vivo device according to claim 1, wherein the detecting reagent comprises an antibody.

5. The in vivo device according to claim 1, wherein the detecting reagent is immobilized in the reaction chamber.

6. The in vivo device according to claim 1 wherein the waste chamber is vacuum-packed.

7. The in vivo device according to claim 1 wherein a pressure gradient between the waste chamber and the reaction chamber facilitates flow when the gate is opened.

8. The in vivo device according to claim 1 wherein the waste chamber includes an absorbing agent and a vent.

9. The in vivo device according to claim 1 wherein the gate is sensitive to pH, temperature or electric current.

10. The in vivo device according to claim 1 wherein the reaction chamber is embedded in a multi-layered substrate.

11. The in vivo device according to claim 1 wherein the gate includes a silicon nitrite layer and a heating element.

12. The in vivo device according to claim 1 comprising a sample chamber fluidically connected to the reaction chamber, wherein the sample chamber includes a dry buffer.

13. The in vivo device according to claim 1 comprising a washing solution chamber fluidically connected to the reaction chamber, wherein the washing solution chamber includes washing solution to wash the reaction chamber.

14. The in vivo device according to claim 1 wherein the conjugated antibody is integrated into a liposome.

15. The in vivo device according to claim 1 wherein the in vivo device is an autonomous ingestible capsule.

16. A method for detecting the presence of an analyte in an in vivo fluid sample, the method comprising:

collecting the in vivo fluid sample into a detection system in vivo, wherein the detection system comprises;

an inlet for obtaining the fluid sample in vivo;

a reaction chamber in which a detecting reagent reacts with the analyte in the fluid sample;

a waste chamber;

a gate to fluidically connect the reaction chamber to the waste chamber;

detecting a reaction between an analyte in the fluid sample and the detecting reagent; and

imaging the reaction chamber.

17. The method according to claim 16 wherein the detecting reagent is conjugated to an optically detectable particle.

18. The method according to claim 17 wherein the detectable particle is a colored polymer particle or a particle with fluorescence properties.

19. A system to detect the presence of an analyte in a fluid sample comprising:

an in vivo device comprising;

an inlet for obtaining the fluid sample in vivo;

a reaction chamber in which a detecting reagent reacts with the analyte in the fluid sample;

a waste chamber;

a gate to fluidically connect the reaction chamber to the waste chamber;

an imager for imaging in vivo and a portion of the reaction chamber; and

a transmitter for transmitting image data;

an external receiver device;

an external processor with a display unit;

wherein the external processor is configured to display images obtained by the imager.

20. The system according to claim 19, wherein the fluid sample is of the GI tract.

21. An in-vivo imaging device comprising:

a housing said housing comprising a window;

an illumination source located within the housing to illuminate a body lumen through the window;

an imager to receive light reflected from the body lumen through the window; and

a transmitter to transmit image data to a receiving system;

wherein the window is coated with liposomes containing a marker.

22. The imaging device according to claim 21 wherein the housing is capsule shaped.

23. The imaging device according to claim 21 wherein the imager is to acquire images including the marking.