Substrates for immobilizing cells and tissues and methods of use thereof

• US 20080153077A1
• Filed: 06/08/2007
• Published: 06/26/2008
• Est. Priority Date: 06/12/2006
• Status: Abandoned Application

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Henry, David
• Application Number:
  US11/811,099
• Publication Number:
  US 20080153077A1
• Time in Patent Office: Days
• US Class Current: 435/1.1
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

Described herein are substrates for immobilizing cells and tissues and methods of use thereof.

Claims

1. A substrate for immobilizing cells or tissue comprising:

a. a network of nanofibers, and

b. a base substrate comprising a non-woven or woven porous substrate, wherein the base substrate comprises a first outer surface, wherein the network of nanofibers is adjacent to the first outer surface of the base substrate.

2. The substrate of claim 1, wherein the network of nanofibers has a pore size less than the diameter of the cell or tissue.

3. The substrate of claim 1, wherein the base substrate has a pore size less than the diameter of the cell or tissue.

4. The substrate of claim 1, wherein the network of fibers has a pore size greater than 0.2 μ

m.

5. The substrate of claim 1, wherein the network of fibers has a pore size less than 1 μ

m.

6. The substrate of claim 1, wherein the base substrate has a pore size greater than 0.2 μ

m.

7. The substrate of claim 1, wherein the network of nanofibers comprises electrospun natural or synthetic fibers.

8. The substrate of claim 1, wherein the network of nanofibers comprises a polyolefin, cyclic polyolefin, polyacetal, polyamide, polyester, polycarbonate, cellulose ether and ester, polyalkylene sulfide, polyarylene oxide, polyalkylene oxide, copolymers and block copolymers of alkylene oxide, polyvinylcarbazole, polysulfone, a modified polysulfone polymer, or a mixture thereof.

9. The substrate of claim 1, wherein the base substrate comprises a polyolefin, cyclic polyolefin, polyacetal, polyamide, polyester, polycarbonate, cellulose ether and ester, polyalkylene sulfide, polyarylene oxide, polyalkylene oxide, copolymers and block copolymers of alkylene oxide, polyvinylcarbazole, polysulfone, a modified polysulfone polymer, or a mixture thereof.

10. The substrate of claim 1, wherein the base substrate comprises a polyamide.

11. The substrate of claim 1, wherein the network of nanofibers comprises a polylactide-co-glycolide and the base substrate comprises a woven substrate comprising polyamide.

12. The substrate of claim 1, wherein the network of nanofibers, the base substrate, or a combination thereof further comprises one or more bioactive molecules.

14. A method for growing a plurality of cells, comprising (a) depositing a parent set of cells on the substrate of claim 1, and (b) culturing the substrate with the deposited cells to promote the growth of the cells.

15. A method for differentiating cells, comprising (a) depositing a parent set of cells on the substrate of claim 1, and (b) culturing the substrate to promote differentiation of the cells.

16. A method for determining an interaction between a known cell line and a drug, comprising (a) depositing the known cell line on the substrate of claim 1;

(b) contacting the deposited cells with the drug; and

(c) identifying a response produced by the deposited cells upon contact with the drug.

17. A method for separating a compound present in a solution, comprising (a) contacting the substrate of claim 1 with the solution, wherein the compound is immobilized on the substrate;

and (b) removing the immobilized compound from the substrate.

18. A method for growing tissue, comprising (a) depositing a parent set of cells that are a precursor to the tissue on the substrate of claim 1, and (b) culturing the substrate with the deposited cells to promote the growth of the tissue.

13. A kit comprising one or more networks of nanofibers and base substrate comprising a non-woven porous substrate or a woven porous substrate.