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METHOD AND DEVICE FOR DETECTING THE PRESENCE OF TARGET NUCLEIC ACIDS IN A SAMPLE, AND MICROFLUIDIC DEVICE FOR USE IN SUCH METHODS

  • US 20080153091A1
  • Filed: 08/13/2007
  • Published: 06/26/2008
  • Est. Priority Date: 04/17/1997
  • Status: Abandoned Application
First Claim
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1. A method for detecting whether at least one molecule of a first target nucleic acid is present in a sample and detecting whether at least one molecule of a second target nucleic acid is present in said sample, said method comprising:

  • loading a first portion of said sample into at least a first sample chamber, whereby if said first portion contains at least a single molecule of said first target nucleic acid, said first portion would attain a detectable concentration of said first target nucleic acid within a portion of said first sample chamber after a single round of amplification;

    loading a second portion of said sample into at least a second sample chamber, whereby if said second portion contains at least a single molecule of said second target nucleic acid, said second portion would attain a detectable concentration of said second target nucleic acid within a portion of said second sample chamber after a single round of amplification;

    subjecting said first portion in said first sample chamber to at least a first amplification step;

    subjecting said second portion in said second sample chamber to at least a first amplification step; and

    thendetermining whether said first portion contains at least one molecule of said first target nucleic acid and determining whether said second sample portion contains at least one molecule of said second target nucleic acid.

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