Articles having localized molecules disposed thereon and methods of producing same

• US 20080153100A1
• Filed: 10/31/2007
• Published: 06/26/2008
• Est. Priority Date: 03/30/2006
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Turner, Stephen, Lyle, John, Roitman, Daniel, Xu, Yue, Korlach, Jonas, Rank, David, Wegener, Jeffery
• Granted Patent: US 9,944,980 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00427 using masks
• CPC Class Codes: B01J 2219/0045 using optical fibres
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00639 the compounds being trapped in or bound to a porous medium
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2527/127 the enzyme inhibitor or activator used
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: Y10T 29/49002 : Electrical device making

Abstract

Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Claims

1-40. -40. (canceled)

41. A method of identifying a sequence of a nucleic acid molecule, comprising:

providing a plurality of nucleic acid polymerase/template/primer complexes within discrete observation regions on a substrate surface, and detecting sequential addition of nucleotides or nucleotide analogs in a template dependent manner to identify a sequence of incorporation of the nucleotides or nucleotide analogs in the plurality of observation regions;

providing an intra-observation region barrier upon the substrate surface between at least first and second discrete observation regions to substantially prevent intra-observation region diffusion of one or more reactants or products.

42. The method of claim 41, wherein the barrier comprises a first fluid and sufficient distance between the first and second observation regions to substantially prevent one or more products or reactants from the first observation region from reaching the second observation region.

43. The method of claim 42, wherein the substrate surface is planar.

44. The method of claim 42, wherein the substrate surface is structured.

45. The method of claim 44, wherein the barrier further comprises at least first and second nanoscale wells disposed in the substrate surface, wherein the first and second observation regions are disposed within the first and second nanoscale wells, respectively.

46. A method of preferentially localizing desired molecules within an optical confinement disposed upon a substrate, comprising:

depositing the desired molecules over the surface of the substrate; and

selectively removing the desired molecules from the surface of the substrate that is not within the optical confinement.

47. A method of localizing a molecule within an optical confinement disposed on a substrate, comprising:

providing a light activated coupling group on the surface of the substrate, including within the optical confinement;

directing activating radiation at the substrate, wherein the optical confinement permits activation radiation to be incident only within the optical confinement; and

coupling the molecule to the light activated coupling group.

48. A method of selectively immobilizing a molecule of interest on a substrate, comprising:

providing a substrate having a first surface component and a second surface component where the first and second surface component have different surface characteristics; and

selectively coupling the molecule of interest to the first surface component, based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.

49. The method of claim 48, wherein first surface comprises a SiO2 and the second surface comprises a metal or metal oxide.

50. The method of claim 48, wherein the differing surface characteristics comprise surface charge.

51. The method of claim 48, wherein the molecule of interest associates preferentially to the first surface component.

52. The method of claim 48, wherein the substrate is contacted with an intermediate coupling group that is selective for associating with a surface having the surface characteristics of the first surface component.

53. The method of claim 52, wherein the intermediate binding molecule comprises a phospholipid.

54. A method of selectively depositing a molecule of interest on selected regions of a substrate, comprising:

providing a substrate having first and second components, the first component comprising a conductive material and the second component comprising an insulator; and

applying an electrical potential to the first component so as to increase or decrease association of a molecule of interest with a surface of the first component.